Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TRAPPC6B antibody (STJ191522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-TRAPPC9 antibody (STJA0007028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Tri-Methyl-H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16-Lys21 antibody (STJA0005915)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Tri-Methyl-Histone H3-K27 antibody [2E12#] (STJA0019838)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g TriMethyl-Histone H3-K27 mouse monoclonal antibody antibody (STJ118639) , along with a Goat Anti-mouse IgG (H+L). The CUT&Tag results indicate the enrichment pattern of TriMethyl-Histone H3-K27 in representative gene loci (MYOD1) , as shown in figure.

Anti-Tri-Methyl-Histone H3-K27 antibody [S9MM] (STJ118639)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat/Other

£143.50 - £618.50

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Anti-Tri-Methyl-Histone H3-K9 antibody [SAA1473] (STJA0019839)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Rabbit polyclonal antibody (STJ23972). Blue: DAPI for nuclear staining.

Anti-Tri-Methyl-Histone H4-K20 antibody (STJ23972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H4-Lys20 antibody (STJ197281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Tri-Methyl-Histone H4-Lys59 antibody (STJ197280)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Tri-Methyl-Histone H4-Lys79 antibody (STJ197279)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Tri-Methyl-TP53-Lys370 antibody (STJA0005917)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using TRIAP1 rabbit polyclonal antibody (STJ29452) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TRIAP1 antibody (1-76) (STJ29452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-TRIAP1 antibody (STJA0007880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts from wild type (WT) and TRIM25 knockout (KO) 293T (KO) cells, using TRIM25 antibody (STJ11102049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TRIM25 antibody (100-200) [S9MR] (STJ11102049)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various lysates, using TRIM25 rabbit polyclonal antibody (STJ114804) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TRIM25 antibody (190-630) (STJ114804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-TRIM25 antibody (201-250 aa) (STJ92843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-TRIM25 antibody [R06-1H2] (STJA0034953)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-TRIM25 antibody [SRM14853] (STJ11109583)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemistry analysis of TRIM27 in paraffin-embedded Mouse kidney using TRIM27 Rabbit polyclonal antibody (STJ118505) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TRIM27 antibody (1-200) (STJ118505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TRIM27 antibody (34-84 aa) (STJ193470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using KAP1/TRIM28 antibody (STJ11101703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TRIM28 antibody (250-350) [S3MR] (STJ11101703)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug KAP1/TRIM28 Rabbit polyclonal antibody (STJ25965 1:400). Western blot was performed from the immunoprecipitate using KAP1/TRIM28 Rabbit polyclonal antibody (STJ25965) at a dilition of 1:1000.

Anti-TRIM28 antibody (576-835) (STJ25965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot detection of KAP1/TIF1 beta in 293T and HepG2 cell lysates using KAP1/TIF1 beta mouse mAb (1:1000 diluted).Observed band size: 110KDa.

Anti-TRIM28 antibody [4E1-D12-F8] (STJ99057)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human

£149.00 - £360.00

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Anti-TRIM28 IP antibody [SAA0249] (STJA0018764)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£195.50 - £1,319.50

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Anti-TRIM28 nanobody [SAA1235] (STJN000263)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Immunofluorescence analysis of U2OS cells using TRIM4 Rabbit polyclonal antibody (STJ118367) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-TRIM4 antibody (1-280) (STJ118367)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-TriMethyl-Histone H3-Lys27 antibody (STJA0033012)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/ChIP

Reactivity :

Human

£224.50 - £319.50

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Anti-TriMethyl-Histone H3-Lys27 antibody [R01-8I4] (STJA0035048)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP/ChIP

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-TriMethyl-Histone H3-Lys36 antibody [7G6-8F3-3H9] (STJA0032853)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-TriMethyl-Histone H3-Lys79 antibody [6A6-5D1-10A10] (STJA0032789)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-TriMethyl-Histone H3-Lys79 antibody [8G4-3D10-6D4] (STJA0032855)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-TriMethyl-Histone H3-Lys79 antibody [9G4-10G7-4A1] (STJA0032854)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-TriMethyl-Histone H3-Lys9 antibody (STJA0037473)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-TriMethyl-Histone H4-K20 antibody [SRM14965] (STJ11109695)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£118.50 - £521.50

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Anti-TriMethyl-Histone H4-Lys20 antibody (STJA0033018)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£224.50 - £319.50

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Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells using 3 Mu g TRIP4 antibody (STJ117547). Western blot was performed from the immunoprecipitate using TRIP4 antibody (STJ117547) at a dilution of 1:1000.

Anti-TRIP4 antibody (282-581) (STJ117547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from K562 cells, using TRIP4 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-TRIP4 antibody (81-130 aa) (STJ91731)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using TRMT112 Rabbit polyclonal antibody (STJ116522) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TRMT112 antibody (1-125) (STJ116522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TRMT112 antibody (65-115 aa) (STJ195606)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of TRRAP in paraffin-embedded human esophageal cancer using TRRAP Rabbit polyclonal antibody (STJ11101534) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TRRAP antibody (3660-3859) (STJ11101534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TRRAP antibody (STJ191546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Tryptophan tRNA Ligase antibody [R01-4H2] (STJA0037241)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Tryptophan tRNA Ligase antibody [R09-1D2] (STJA0033792)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Immunofluorescence analysis of U-2 OS cells using TSFM Rabbit polyclonal antibody (STJ118534) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TSFM antibody (245-346) (STJ118534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TSFM antibody (STJA0008641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TSFM antibody [PT0800R] (STJA0039361)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

£150.00 - £540.00

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Anti-TSFM antibody [SRM13118] (STJ11107848)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Thyroid Stimulating Hormone antibody IHC human pituitary adenoma STJ180625

Anti-TSH antibody [ZM294] (STJ180625)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£146.50 - £523.50

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