• Immunofluorescence analysis of U-2 OS cells using TSFM Rabbit polyclonal antibody (STJ118534) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using TSFM Rabbit polyclonal antibody (STJ118534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TSFM antibody (245-346) (STJ118534)

SKU:
STJ118534

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TSFM (245-346) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSFM
Gene ID: 10102
Uniprot ID: EFTS_HUMAN
Immunogen Region: 245-346
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 245-346 of human TSFM (NP_001166167.1).
Immunogen Sequence: PSLHKLVLGKYGALVICETS EQKTNLEDVGRRLGQHVVGM APLSVGSLDDEPGGEAETKM LSQPYLLDPSITLGQYVQPQ GVSVVDFVRFECGEGEEAAE TE
Tissue Specificity Expressed in all tissues, with the highest levels of expression in skeletal muscle, liver and kidney.
Function Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
Protein Name Elongation Factor Ts - Mitochondrial
Ef-Ts
Ef-Tsmt
Database Links Reactome: R-HSA-5389840
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Elongation Factor Ts - Mitochondrial antibody
Anti-Ef-Ts antibody
Anti-Ef-Tsmt antibody
Anti-TSFM antibody

Information sourced from Uniprot.org

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