Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NFKBIA-Ser32 antibody (STJ194713)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of rat-spleen tissue. 1, I Kappa B-Alpha (phospho Ser32/S36) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-NFKBIA-Ser32/S36 antibody (15-64 aa) (STJ90311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

Choose Options

Anti-Phospho-NFKBIA-Tyr-305 antibody (STJA0005444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£365.50

Choose Options

Anti-Phospho-NFKBIA-Tyr-42 antibody (STJA0005443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£365.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human lymph node, using IkappaB-alpha (Phospho-Tyr305) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NFKBIA-Tyr305 antibody (268-317 aa) (STJ90912)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NFKBIA-Tyr42 antibody (9-58 aa) (STJ90312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from HeLa cellss, using Phospho-I Kappa B Alpha-Y42 Rabbit pAb (STJ22317). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-NFKBIA-Y42 antibody (STJ22317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£126.50 - £618.50

Choose Options

Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NPM1-Ser4 antibody (STJ194732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Western Blot analysis of RAW 264. 7 cell, Raji cell, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NPM1-Ser70 antibody (STJA0010019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of various lysates, using Phospho-NPM1-T199 Rabbit pAb (STJ11100977) at 1:900 dilution. HeLa cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-NPM1-T199 antibody (STJ11100977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

Choose Options

Western blot analysis of B23 (phospho Thr199) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with forskolin 40nM 30', 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-NPM1-Thr199 antibody (171-220 aa) (STJ91062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-NPM1-Thr234 antibody (201-250 aa) (STJ90546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NPM1-Thr95 antibody (STJ194733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Glucocorticoid Receptor-S211 Rabbit pAb (STJ116380) at 1:600 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-NR3C1-S211 antibody (STJ116380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

Choose Options

Western blot analysis of A549 cells using Phospho-GR (S203) Polyclonal Antibody diluted at 1：500

Anti-Phospho-NR3C1-Ser203 antibody (171-220 aa) (STJ90572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

Choose Options

Western blot analysis of various cells using Phospho-GR (S211) Polyclonal Antibody

Anti-Phospho-NR3C1-Ser211 antibody (181-230 aa) (STJ90761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of HELA 293T cells using Phospho-GR (S226) Polyclonal Antibody diluted at 1：500

Anti-Phospho-NR3C1-Ser226 antibody (201-250 aa) (STJ90794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NR5A1-Ser203 antibody (STJA0009976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-Phospho-NRF2-S40 antibody [SRM13683] (STJ11108413)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

Add to Cart The item has been added

Anti-Phospho-NRF2-S40-S40 antibody [SRM15139] (STJ11109869)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

Add to Cart The item has been added

Anti-Phospho-Nrf2-Ser40 antibody [R03-4A1] (STJA0036231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human

£133.50 - £281.50

Choose Options

Anti-Phospho-Nrf2-Ser40 antibody [R03-7C4] (STJA0033908)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

£133.50 - £281.50

Choose Options

Anti-Phospho-Nucleophosmin-Thr199 antibody [R01-2D6] (STJA0035082)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Rat

£133.50 - £281.50

Choose Options

Anti-Phospho-OGT-Thr454 antibody (STJA0009873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-Phospho-p53-S15-S15 antibody [SRM15140] (STJ11109870)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£0.00

Add to Cart The item has been added

Anti-Phospho-p53-Ser15 antibody [PT0901R] (STJA0039935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

Choose Options

Anti-Phospho-p53-Ser33 antibody [R04-1B1] (STJA0035772)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

Choose Options

Anti-Phospho-p53-Ser376 antibody [R06-1Y5] (STJA0035935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

Choose Options

Anti-Phospho-p53-Ser392 antibody (STJA0037463)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

Choose Options

Anti-Phospho-p53-Ser392 antibody [PT1223R] (STJA0040171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

Choose Options

Anti-Phospho-p53-Ser392 antibody [R08-2A8] (STJA0035832)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

Choose Options

Anti-Phospho-p53-Ser46 antibody [PT0903R] (STJA0039796)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

Choose Options

Anti-Phospho-p53-Ser6 antibody [PT1249R] (STJA0040157)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

Choose Options

Anti-Phospho-p53-Ser6 antibody [R02-0F2] (STJA0035083)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

Choose Options

Anti-Phospho-p53-Ser9 antibody [R03-4Y8] (STJA0035775)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

Choose Options

Anti-Phospho-p53-T18 antibody [23#] (STJA0019168)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

Choose Options

Western blot analysis of lysates from C6 cells, using Phospho-p53-T81 Rabbit pAb (STJ11103770) at 1:500 dilution. C6 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-p53-T81 antibody (STJ11103770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

Choose Options

Anti-Phospho-p53-Thr55 antibody [R06-4R5] (STJA0035934)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

Choose Options

Western blot of Jurkat cell lysate showing specific immunolabeling of the ~48 kDa p62 phosphorylated at Ser28 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-p62-Ser28 antibody (STJA0003736)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

£488.50

Choose Options

Western blot of Jurkat cell lysate showing specific immunolabeling of the ~62 kDa p62 phosphorylated at Thr269/Ser272 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-p62-Thr269/Ser272 antibody (STJA0003737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

£488.50

Choose Options

Western blot of HEK293 cells transfected with Parkin wild type (WT) and Parkin S101 mutant (Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser101.

Anti-Phospho-PARK2-Ser101 antibody (STJA0003742)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Non-Human Primates

£488.50

Choose Options

Western blot of HEK293 cells transfected with Parkin wild type (WT) and Parkin S378 mutant (Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser378.

Anti-Phospho-PARK2-Ser378 antibody (STJA0003743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Non-Human Primates

£488.50

Choose Options

Western blot analysis of various lysates using Phospho-PDHA1-S293 Rabbit pAb (STJ11103767) at 1:1000 dilution. 293T cells and NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PDHA1-S293 antibody (STJ11103767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb (STJ11102604) at 1:23000 dilution. 293T cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PDHA1-S293 antibody [S4MR] (STJ11102604)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

Choose Options

Western blot analysis of extracts of HeLa cells, using Phospho-Tau-T181 antibody (STJ11105820) at1:20000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-PDHA1-S293 antibody [S5820RM] (STJ11105820)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£143.50 - £618.50

Choose Options

Anti-Phospho-PDHA1-Ser293 antibody [R46-5D-6] (STJA0035679)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

Choose Options

Western Blot analysis of 1 HepG2 cell 2, LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PDHA1-Ser293/291 antibody (STJA0009914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PDK1-Thr338 antibody (STJA0009880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options