• Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NFKBIA-Ser32 antibody (STJ194713)

SKU:
STJ194713

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-NF-kappa-B inhibitor alpha-Ser32 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFKBIA
Gene ID: 4792
Uniprot ID: IKBA_HUMAN
Specificity: This antibody detects endogenous levels of Human Mouse Rat I Kappa B Alpha (phospho-Ser32)
Immunogen: Synthesized phosho peptide around human I Kappa B Alpha (Ser32)
Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Protein Name Nf-Kappa-B Inhibitor Alpha
I-Kappa-B-Alpha
Ikb-Alpha
Ikappabalpha
Major Histocompatibility Complex Enhancer-Binding Protein Mad3
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1810476
Reactome: R-HSA-202424
Reactome: R-HSA-209560
Reactome: R-HSA-2871837
Reactome: R-HSA-445989
Reactome: R-HSA-4755510
Reactome: R-HSA-5603029
Reactome: R-HSA-5607764
Reactome: R-HSA-5689880
Reactome: R-HSA-9020702
Reactome: R-HSA-933542
Reactome: R-HSA-9692916
Cellular Localisation Cytoplasm
Nucleus
Shuttles Between The Nucleus And The Cytoplasm By A Nuclear Localization Signal (Nls) And A Crm1-Dependent Nuclear Export
Alternative Antibody Names Anti-Nf-Kappa-B Inhibitor Alpha antibody
Anti-I-Kappa-B-Alpha antibody
Anti-Ikb-Alpha antibody
Anti-Ikappabalpha antibody
Anti-Major Histocompatibility Complex Enhancer-Binding Protein Mad3 antibody
Anti-NFKBIA antibody
Anti-IKBA antibody
Anti-MAD3 antibody
Anti-NFKBI antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance