Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using p38 MAPK antibody (STJ112728) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-MAPK14 antibody (261-360) (STJ112728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €742.20

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Immunoprecipitation analysis of 200 Mu g extracts of U-87MG cells, using 3 Mu g p38 MAPK antibody (STJ116613). Western blot was performed from the immunoprecipitate using p38 MAPK antibody (STJ116613) at a dilution of 1:1000.

Anti-MAPK14 antibody (261-360) (STJ116613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Whole cell lysates of HepG2 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-p38 (PTR2306) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2Predicted band size: 41kDaObserved band size: 38kDa

Anti-MAPK14 antibody (300-360 aa) [4G2] (STJ196672)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€216.00 - €313.20

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Western blot analysis of HELA KB PC-3 using p38 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-MAPK14 antibody (C-Term) (STJ97615)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

€58.80 - €313.20

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Anti-Mapk14 antibody (C-Term) [M548] (STJA0005504)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€383.40

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-MAPK14 antibody (STJ99619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various lysates using ERK1 Rabbit monoclonal antibody (STJ11104593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-MAPK3 antibody (1-100) [S4593RM] (STJ11104593)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€151.80 - €665.40

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Western blot analysis of various lysates, using ERK1 antibody (STJ11101698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK3 antibody (1-100) [S8MR] (STJ11101698)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Immunohistochemistry analysis of ERK1 in paraffin-embedded mouse heart using [KD Validated] ERK1 Rabbit polyclonal antibody (STJ24487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAPK3 antibody (1-378) (STJ24487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of various cells using ERK 1 Polyclonal Antibody diluted at 1：500

Anti-MAPK3 antibody (161-210 aa) (STJ92987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunofluorescence analysis of PC-12 using ERK1 Rabbit monoclonal antibody (STJ11104626) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK3 antibody (200-300) [S4626RM] (STJ11104626)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Rat

€151.80 - €665.40

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Western blot with Anti-MAPK3 Antibody (STJ501702) of rat testis at 1:250 dilution in 1% milk solution.

Anti-MAPK3 antibody (300-350) (STJ501702)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€480.60

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Anti-MAPK3 antibody (300-350) {Biotin} (STJ501703)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€597.00

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Anti-MAPK3 antibody (300-350) {FITC} (STJ501704)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€597.00

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Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-MAPK3 antibody (STJ197394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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SP1-Induced Upregulation of LncRNA AFAP1-AS1 Promotes Tumor Progression in Triple-Negative Breast Cancer by Regulating STJ195658

Anti-MAPKAP1 antibody (193-243 aa) (STJ195658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of MAPKAP1 in paraffin-embedded rat kidney using MAPKAP1 Rabbit polyclonal antibody (STJ117829) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAPKAP1 antibody (360-522) (STJ117829)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western blot analysis of lysates from MCF-7 cells, using MAPKAPK5 Antibody. The lane on the right was blocked with the STJ95213

Anti-MAPKAPK5 antibody (148-197 aa) (STJ95213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis using PRAK monoclonal antibody against truncated PRAK recombinant protein.

Anti-MAPKAPK5 antibody [7H10B4] (STJ98335)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

€178.80 - €432.00

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Anti-Mature-BDNF antibody (C-Term) (STJ13100057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse/Marmoset

€387.00

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Anti-Mature-BDNF antibody (C-Term) (STJ13100064)

Clonality :

Polyclonal

Host :

Guinea Pig

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse/Marmoset

€387.00

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Anti-Mature-BDNF antibody (Internal Region) (STJ13100056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

€387.00

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Western blot analysis of extracts of Mouse liver cells, using MBD2 antibody (STJ11103547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-MBD2 antibody (100-200) [S7MR] (STJ11103547)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€142.20 - €625.80

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C overnight STJ195192

Anti-MBD2 antibody (110-160 aa) (STJ195192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of extracts of various cell lines, using MBD2 antibody (STJ24510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MBD2 antibody (202-411) (STJ24510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€132.60 - €606.60

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Western blot analysis of lysates from mouse heart, using MBD2 Rabbit pAb (STJ11106052) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-MBD2 antibody (350-411 aa) (STJ11106052)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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MBD2 antibody western blot A431 cells STJA0037083

Anti-MBD2 antibody [R03-2N2] (STJA0037083)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

€160.20 - €337.80

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Anti-MBD2 antibody [R05-4G5] (STJA0034546)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€168.60 - €370.20

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Western blot analysis of various lysates using MBD3 Rabbit mAb (STJ11102393) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-MBD3 antibody (1-100) [S3MR] (STJ11102393)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Western blot analysis of various lysates using MBD3 Rabbit polyclonal antibody (STJ24511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MBD3 antibody (1-255) (STJ24511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€132.60 - €606.60

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Western Blot analysis of various cells using MBD3 Polyclonal Antibody STJ94036

Anti-MBD3 antibody (221-270 aa) (STJ94036)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

€58.80 - €313.20

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MBD3 antibody western blot 293T cells STJA0036776

Anti-MBD3 antibody [R05-8G9] (STJA0036776)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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MBD3 antibody western blot HeLa lysates STJA0037459

Anti-MBD3 antibody [R06-6D4] (STJA0037459)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MBIP antibody (1-80 aa) (STJ190254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Anti-MCRS1 antibody (STJA0007092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of various lysates using MDC1 Rabbit pAb (STJ110656) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 5s.

Anti-MDC1 antibody (1-350) (STJ110656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

€132.60 - €606.60

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Immunohistochemistry analysis of MDM2 in paraffin-embedded human lung squamous carcinoma tissue using MDM2 Rabbit monoclonal antibody (STJ11105095) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-MDM2 antibody (101-200) [S5095RM] (STJ11105095)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

€142.20 - €625.80

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Western blot analysis of lysates from U2OS cells, using MDM2 Rabbit monoclonal antibody (STJ11105096) at 1:2000 dilution. U2OS cells were treated by Nutlin 3a (10 Mu M) at 37 °C for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MDM2 antibody (101-200) [S5096RM] (STJ11105096)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€142.20 - €625.80

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Immunofluorescence analysis of Hela cell. 1, MDM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3B Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MDM2 antibody (132-181 aa) (STJ94057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

€58.80 - €313.20

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Immunofluorescence analysis of NIH/3T3 cells, using MDM2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MDM2 antibody (151-200 aa) (STJ94056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Anti-MDM2 antibody IHC Human atypical lipomatous tumor STJ180166

Anti-MDM2 antibody (154-167aa) [SMP14] (STJ180166)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

€178.20 - €687.00

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Western blot analysis of C2C12, using MDM2 antibody (STJ115290) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MDM2 antibody (200-430) (STJ115290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Immunohistochemistry analysis of paraffin-embedded human urothelial carcinoma using MDM2 Rabbit polyclonal antibody (STJ24533) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-MDM2 antibody (231-330) (STJ24533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

€132.60 - €606.60

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Immunofluorescence analysis of human-liver-cancer tissue. 1, MDM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-MDM2 antibody (381-430 aa) (STJ96551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Immunofluorescence analysis of A549 cells, using MDM2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MDM2 antibody (391-440 aa) (STJ94055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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MDM2 antibody immunohistochemistry dedifferentiated liposarcoma paraffin-embedded STJA0037706

Anti-MDM2 antibody [BP6203] (STJA0037706)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

€370.20

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Anti-MDM2 antibody [IHC620] (STJA0042211)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

€157.80 - €677.40

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Anti-MDM2 antibody [PT0681R] (STJA0039292)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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