Anti-MBD2 antibody (202-411) (STJ24510)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MBD2 (202-411) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MBD2 |
| Gene ID | 8932 |
| Uniprot ID | MBD2_HUMAN |
| Immunogen Region | 202-411 |
| Immunogen Sequence | DLSSFDFRTGKMMPSKLQKN KQRLRNDPLNQNKGKPDLNT TLPIRQTASIFKQPVTKVTN HPSNKVKSDPQRMNEQPRQL FWEKRLQGLSASDVTEQIIK TMELPKGLQGVGPGSNDETL LSAVASALHTSSAPITGQVS AAVEKNPAVWLNTSQPLCKA FIVTDEDIRKQEERVQQVRK KLEEALMADILSRAADTEEM DIEMDSGDEA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 202-411 of human MBD2 (NP_003918.1). |
Additional Info
| Tissue Specificity | Highly expressed in brain, heart, kidney, stomach, testis and placenta. |
| Function | Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases to chromatin. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Acts as a transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters. |
| Protein Name | Methyl-Cpg-Binding Domain Protein 2DemethylaseDmtaseMethyl-Cpg-Binding Protein Mbd2 |
| Database Links | Reactome: R-HSA-427413Reactome: R-HSA-73728 |
| Cellular Localisation | NucleusChromosomeNuclearIn Discrete FociDetected At Replication Foci In Late S PhaseLocalizes To Methylated ChromatinLocalizes To Sites Of Dna Damage In A Manner Partially Dependent On Zmynd8 |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Domain Protein 2 antibodyAnti-Demethylase antibodyAnti-Dmtase antibodyAnti-Methyl-Cpg-Binding Protein Mbd2 antibodyAnti-MBD2 antibody |
Information sourced from Uniprot.org