Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of various lysates using NUP160 Rabbit polyclonal antibody (STJ115047) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 180s.

Anti-NUP160 antibody (40-150) (STJ115047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates using NUP205 Rabbit polyclonal antibody (STJ11100381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NUP205 antibody (10-120) (STJ11100381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and NUP210 knockout (KO) 293T cells, using [KO Validated] NUP210 Rabbit polyclonal antibody (STJ11100771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NUP210 antibody (680-880) (STJ11100771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of U-2 OS cells using NUP210 Rabbit polyclonal antibody (STJ11104329) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NUP210 antibody (680-880) (STJ11104329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates using NUP214 Rabbit pAb (STJ110655) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-NUP214 antibody (750-1030) (STJ110655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using NUP35 Rabbit polyclonal antibody (STJ114635) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NUP35 antibody (1-326) (STJ114635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using NUP50 Rabbit pAb (STJ111702) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-NUP50 antibody (340-468) (STJ111702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using NUP54 Rabbit polyclonal antibody (STJ117649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NUP54 antibody (100-400) (STJ117649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using NUP62 Rabbit polyclonal antibody (STJ24850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-NUP62 antibody (403-522) (STJ24850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g NUP62 antibody (STJ11103002). Western blot was performed from the immunoprecipitate using NUP62 antibody (STJ11103002) at a dilution of 1:1000.

Anti-NUP62 antibody (423-522) [S2MR] (STJ11103002)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-NUP62 antibody (N-Term) [M436] (STJA0005499)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£394.50

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Western blot analysis of various lysates using NUP85 Rabbit polyclonal antibody (STJ113234) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NUP85 antibody (427-656) (STJ113234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using NUP93 Rabbit polyclonal antibody (STJ24852) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NUP93 antibody (670-819) (STJ24852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-Occludin antibody (405-522 aa) [SRM] (STJ11107332)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Human placental mesenchymal stromal cells modulate IFN-γ and IL-10 secretion by CD4+T cells via CD73, and alleviate intestinal damage in mice with graft-versus-host disease. INTERNATIONAL IMMUNOPHARMACOLOGY Xiying Luan WB Human, mouse intestine, colon NCM460 cell, Caco-2 cell

Anti-OCLN antibody (350-400 aa) (STJ192969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293T, using Occludin antibody (STJ114495) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-OCLN antibody (350-450) (STJ114495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of Mouse lung, using Occludin Rabbit polyclonal antibody (STJ111161) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-OCLN antibody (400-522) (STJ111161)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-OCLN antibody at 1:1000 dilution lysates at 50 µg per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-OCLN antibody (500aa C-Term) (STJ140166)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Anti-OCLN antibody (AA range: 1-522) [PTR1394] (STJA0038890)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-OCLN antibody [PT0483R] (STJA0039506)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Human lymph node stained with anti-OCT-2 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of follicular center B cells.

Anti-OCT-2 antibody (112-297aa) [ZM90] (STJ180327)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£178.50 - £685.50

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Anti-Oct-2 antibody [SM5046] (STJA0005046)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Western blot analysis of HEPG2 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-OGT antibody (435-475 aa) (STJ99662)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 cells using OGT Rabbit polyclonal antibody (STJ24862) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-OGT antibody (510-699) (STJ24862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using OGT Rabbit pAb (STJ111128) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-OGT antibody (857-1036) (STJ111128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Chromatin immunoprecipitation analysis of extracts of 293T cells, using OGT Rabbit monoclonal antibody antibody (STJ11101887) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-OGT antibody (947-1046) [S7MR] (STJ11101887)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot with Anti-OGT Antibody (STJ501999) of mouse tetis at 1:500 dilution in buffer.

Anti-OGT antibody (980-1030) (STJ501999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-OGT antibody (980-1030) {Biotin} (STJ502000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-OGT antibody (980-1030) {FITC} (STJ502001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-OGT antibody [PT1337R] (STJA0040061)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-OPN/SPP1 antibody [SAA0512] (STJA0027662)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/SPR/WB

Reactivity :

Human

£254.50 - £378.50

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Anti-OPN/SPP1 antibody [SAA0512] {APC} (STJA0029117)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human

£310.50 - £472.50

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Anti-OPN/SPP1 antibody [SAA0512] {FITC} (STJA0028151)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human

£254.50 - £378.50

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Anti-OPN/SPP1 antibody [SAA0512] {PerCP} (STJA0030083)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human

£292.50 - £440.50

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Anti-OPN/SPP1 antibody [SAA0512] {PE} (STJA0031049)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human

£310.50 - £472.50

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Western blot analysis of various lysates using OPRK1 Rabbit pAb (STJ110604) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-OPRK1 antibody (1-60) (STJ110604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using OPRK1 antibody (STJ115970) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-OPRK1 antibody (280-380) (STJ115970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from COS7 cells, using OPRK1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-OPRK1 antibody (321-370 aa) (STJ93853)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-OPRK1 antibody (C-Term) (STJA0005799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£455.50

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Anti-OPRK1 nanobody [SAA1169] (STJN000215)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using OPRM1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-OPRM1 antibody (21-70) (STJ94182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using MOR-1 Polyclonal Antibody diluted at 1：2000

Anti-OPRM1 antibody (341-390) (STJ94181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of mouse brain using Mu Opioid Receptor (MOR) Rabbit polyclonal antibody (STJ11101441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-OPRM1 antibody (354-388) (STJ11101441)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-OPRM1 antibody (354-388) [S4075RM] (STJ11104075)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Mouse

£129.50 - £574.50

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Immunohistochemistry with Anti-OPRM1 Antibody (STJ501771) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000B. FFPE section.

Anti-OPRM1 antibody (405-455) (STJ501771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-OPRM1 antibody (405-455) {Biotin} (STJ501772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-OPRM1 antibody (405-455) {FITC} (STJ501773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-OPRM1 antibody (C-Term) (STJA0005803)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£455.50

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