Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of extracts of 293T cells, using Phospho-Beta-Catenin-S33/S37 rabbit monoclonal antibody (STJ11102571) at 1:1000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-Phospho-CTNNB1-S33/S37 antibody [S1MR] (STJ11102571)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Beta-Catenin-S33/S37/T41 antibody (STJ11100967) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-CTNNB1-S33/S37/T41 antibody (STJ11100967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of HeLa cells, using Phospho-Beta-Catenin-S45 antibody (STJ29346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-CTNNB1-S45 antibody (STJ29346)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of various cell lines, using Phospho-beta Catenin-S552 antibody (STJ29345) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37°C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37°C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-Phospho-CTNNB1-S552 antibody (STJ29345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts from NIH/3T3 cells, using Phospho-Beta-Catenin-S552 antibody (STJ11105815) at 1000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-CTNNB1-S552 antibody [S5815RM] (STJ11105815)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of extracts of C6 cells, using Phospho-Beta-Catenin-S675 antibody (STJ117893) at 1:1000 dilution. C6 cell lysates were treated by CIP at 37°C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-CTNNB1-S675 antibody (STJ117893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Catenin-beta (Phospho-Ser33) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CTNNB1-Ser33 antibody (17-66 aa) (STJ90206)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of human-liver-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-CTNNB1-Ser37 antibody (3-52 aa) (STJ90207)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts from NIH-3T3 cells, using Phospho-Catenin-Beta (S552) Polyclonal Antibody.

Anti-Phospho-CTNNB1-Ser552 antibody (STJ91364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CTNNB1-Ser675 antibody (STJ196478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts from SW626 cells using Phospho-Beta-Catenin-T41/S45 antibody (STJ22085). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-CTNNB1-T41/S45 antibody (STJ22085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various cells using Phospho-Catenin-Beta (T41/S45) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CTNNB1-Thr41/S45 antibody (11-60 aa) (STJ90208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-CTNNB1-Tyr-142 antibody (STJA0005304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$493.43

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Anti-Phospho-CTNNB1-Tyr-333 antibody (STJA0005305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$493.43

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Anti-Phospho-CTNNB1-Tyr-489 antibody (STJA0005306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$493.43

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Anti-Phospho-CTNNB1-Tyr-654 antibody (STJA0005307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$493.43

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Anti-Phospho-CTNNB1-Tyr-86 antibody (STJA0005303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$493.43

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Western blot analysis of lysates from COS7 cells treated with UV 15', using Catenin-beta (Phospho-Tyr489) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CTNNB1-Tyr489 antibody (455-504 aa) (STJ90928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of 293 cells using Phospho-Catenin-Beta (Y654) Polyclonal Antibody

Anti-Phospho-CTNNB1-Tyr654 antibody (620-669 aa) (STJ90209)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts from 293 cells, using Phospho-Beta-Catenin-Y333 antibody (STJ22086). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-CTNNB1-Y333 antibody (STJ22086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of 293 cells using Phospho-Daxx (S668) Polyclonal Antibody

Anti-Phospho-DAXX-Ser668 antibody (634-683 aa) (STJ90785)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CHOP (Phospho-Ser30) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-DDIT3-Ser30 antibody (15-64 aa) (STJ91075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

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Western blot of jurkat cell lysate showing specific immunolabeling of the ~34 kDa REDD1 protein phosphorylated at Thr23/25 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-DDIT4-Thr23/25 antibody (STJA0003765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

$613.58

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Anti-Phospho-DDX39A-Y138 antibody (STJ11101054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

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Western blot of mouse nuclei lysate showing specific immunolabeling of the ~120 kDa DGCR8 protein phosphorylated at in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-DGCR8-Ser377 antibody (STJA0003611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$613.58

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Immunofluorescence analysis of MCF-7 cells using Phospho-DNMT1-pS714 antibody (STJ22088). Blue: DAPI for nuclear staining.

Anti-Phospho-DNMT1-pS714 antibody (STJ22088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Anti-Phospho-DNMT1-pY399 antibody (STJ22089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Anti-Phospho-DYRK2-Tyr382/264 antibody (STJA0009950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from HeLa cells treated with Etoposide 25uM 24h, using E2F1 (Phospho-Thr433) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-E2F1-Thr433 antibody (388-437 aa) (STJ90458)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts from HUVEC cells, using phospho-EGFR-S1070 antibody (STJ22101). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-EGFR-S1070 antibody (STJ22101)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Anti-Phospho-EGFR-Ser-1142 antibody (STJA0005396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$493.43

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Anti-Phospho-EGFR-Ser-967 antibody (STJA0005397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$493.43

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Western blot analysis of lysates from HeLa cells treated with TSA 400nM 24H, using EGFR (Phospho-Ser1026) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-EGFR-Ser1026 antibody (991-1040 aa) (STJ90567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-EGFR-Ser1036/1037 antibody (STJ196289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Ser1070) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-EGFR-Ser1070 antibody (1041-1090 aa) (STJ90246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from HepG2 cells treated with serum 20% 15', using EGFR (Phospho-Ser1071) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-EGFR-Ser1071 antibody (1036-1085 aa) (STJ90568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 293-EGF cells using Phospho-EGFR (S695) Polyclonal Antibody

Anti-Phospho-EGFR-Ser695 antibody (661-710 aa) (STJ90566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of A-431 and HUVEC cells, using Phospho-EGFR-T669 antibody (STJ22097) at 1:1000 dilution. A431 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. HUVEC cells were treated by EGF. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-EGFR-T669 antibody (STJ22097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-EGFR-T678 antibody (STJ11101148) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-EGFR-T678 antibody (STJ11101148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Anti-Phospho-EGFR-T678 antibody (STJ11107618)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-EGFR-Thr669 antibody (STJ196290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-EGFR (T678) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-EGFR-Thr678 antibody (651-700 aa) (STJ90686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-EGFR (T693) Polyclonal Antibody

Anti-Phospho-EGFR-Thr693 antibody (661-710 aa) (STJ90247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-EGFR-Tyr-1101 antibody [M199] (STJA0005398)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$527.18

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Western blot analysis of HELA cells using Phospho-EGFR (Y1016) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-EGFR-Tyr1016 antibody (986-1035 aa) (STJ91037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-EGFR-Tyr1045 antibody (STJ196291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

$64.80 - $340.20

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Anti-Phospho-EGFR-Tyr1068 antibody (STJ196292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

$64.80 - $340.20

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Western blot analysis of 293 cells using Phospho-EGFR (Y1069) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-EGFR-Tyr1069 antibody (1041-1090 aa) (STJ90515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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