• Immunohistochemical analysis of paraffin-embedded mouse-kidney, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded mouse-kidney, antibody was diluted at 1:100
  • Western blot analysis of Hela cells using Integrin Alpha 5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ITGA5 antibody (561-610 aa) (STJ97284)

SKU:
STJ97284

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Integrin alpha-5 (561-610 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGA5
Gene ID: 3678
Uniprot ID: ITA5_HUMAN
Immunogen Region: 561-610 aa
Specificity: Integrin Alpha 5 Polyclonal Antibody detects endogenous levels of Integrin Alpha 5 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human ITGA5 at the amino acid range 561-610
Post Translational Modifications Proteolytic cleavage by PCSK5 mediates activation of the precursor.
Function Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling. (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Protein Name Integrin Alpha-5
Cd49 Antigen-Like Family Member E
Fibronectin Receptor Subunit Alpha
Integrin Alpha-F
Vla-5
Cd Antigen Cd49e Cleaved Into - Integrin Alpha-5 Heavy Chain - Integrin Alpha-5 Light Chain
Database Links Reactome: R-HSA-1566948
Reactome: R-HSA-1566977
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-445144
Reactome: R-HSA-8941332
Reactome: R-HSA-9634597
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Focal Adhesion
Alternative Antibody Names Anti-Integrin Alpha-5 antibody
Anti-Cd49 Antigen-Like Family Member E antibody
Anti-Fibronectin Receptor Subunit Alpha antibody
Anti-Integrin Alpha-F antibody
Anti-Vla-5 antibody
Anti-Cd Antigen Cd49e Cleaved Into - Integrin Alpha-5 Heavy Chain - Integrin Alpha-5 Light Chain antibody
Anti-ITGA5 antibody
Anti-FNRA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance