• Western blot analysis of extracts of various cell lines, using JARID2 antibody (STJ111865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using JARID2 antibody (STJ111865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-JARID2 antibody (1027-1246) (STJ111865)

SKU:
STJ111865

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-JARID2 (1027-1246) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JARID2
Gene ID: 3720
Uniprot ID: JARD2_HUMAN
Immunogen Region: 1027-1246
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1027-1246 of human JARID2 (NP_004964.2).
Immunogen Sequence: ETVHFATTQWTSMGFETAKE MKRRHIAKPFSMEKLLYQIA QAEAKKENGPTLSTISALLD ELRDTELRQRRQLFEAGLHS SARYGSHDGSSTVADGKKKP RKWLQLETSERRCQICQHLC YLSMVVQENENVVFCLECAL RHVEKQKSCRGLKLMYRYDE EQIISLVNQICGKVSGKNGS IENCLSKPTPKRGPRKRATV DVPPSRLSASSSSKSASSSS
Tissue Specificity During embryogenesis, predominantly expressed in neurons and particularly in dorsal root ganglion cells.
Function Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts as an accessory subunit for the core PRC2 (Polycomb repressive complex 2) complex, which mediates histone H3K27 (H3K27me3) trimethylation on chromatin. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells, thereby playing a key role in stem cell differentiation and normal embryonic development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling. Does not have histone demethylase activity.
Protein Name Protein Jumonji
Jumonji/Arid Domain-Containing Protein 2
Database Links Reactome: R-HSA-212300
Cellular Localisation Nucleus
Colocalizes With The Prc2 Complex On Chromatin
Alternative Antibody Names Anti-Protein Jumonji antibody
Anti-Jumonji/Arid Domain-Containing Protein 2 antibody
Anti-JARID2 antibody
Anti-JMJ antibody

Information sourced from Uniprot.org

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