Anti-ITGA4 antibody (933-1032) [S7MR] (STJ11101987)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Integrin alpha 4 (933-1032) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S7MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:100-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ITGA4 |
| Gene ID | 3676 |
| Uniprot ID | ITA4_HUMAN |
| Immunogen Region | 933-1032 |
| Immunogen Sequence | ALKFEIRATGFPEPNPRVIE LNKDENVAHVLLEGLHHQRP KRYFTIVIISSSLLLGLIVL LLISYVMWKAGFFKRQYKSI LQEENRRDSWSYINSKSNDD |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 933-1032 of human Integrin alpha 4 (ITGA4/CD49d) (P13612). |
Additional Info
| Tissue Specificity | Expressed in vascular smooth muscle cells (at protein level). |
| Post Translational Modifications | Phosphorylation on Ser-1027 inhibits PXN binding. |
| Function | Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. Integrin ITGA4:ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction. |
| Protein Name | Integrin Alpha-4Cd49 Antigen-Like Family Member DIntegrin Alpha-IvVla-4 Subunit AlphaCd Antigen Cd49d |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-202733Reactome: R-HSA-216083Reactome: R-HSA-8949275Reactome: R-HSA-9679191 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Integrin Alpha-4 antibodyAnti-Cd49 Antigen-Like Family Member D antibodyAnti-Integrin Alpha-Iv antibodyAnti-Vla-4 Subunit Alpha antibodyAnti-Cd Antigen Cd49d antibodyAnti-ITGA4 antibodyAnti-CD49D antibody |
Information sourced from Uniprot.org