Anti-ITGA5 antibody (N-Term) (STJ98510)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
ImmunogenPurified recombinant human Integrin Alpha 5 (N-terminus) protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-Integrin alpha-5 (N-Term) for use in WB and FC in Human, Mouse, Rat and Cow samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/FC |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity purification |
| Dilution Range | WB 1:1000-1:2000FC 1:100-1:200 |
| Formulation | Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ITGA5 |
| Gene ID | 3678 |
| Uniprot ID | ITA5_HUMAN |
| Immunogen | Purified recombinant human Integrin Alpha 5 (N-terminus) protein fragments expressed in E.coli. |
| Immunogen Region | N-Term |
| Specificity | Integrin Alpha 5 Monoclonal Antibody detects endogenous levels of Integrin Alpha 5 protein. |
Additional Info
| Post Translational Modifications | Proteolytic cleavage by PCSK5 mediates activation of the precursor. |
| Function | Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling. (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. |
| Protein Name | Integrin Alpha-5Cd49 Antigen-Like Family Member EFibronectin Receptor Subunit AlphaIntegrin Alpha-FVla-5Cd Antigen Cd49e Cleaved Into - Integrin Alpha-5 Heavy Chain - Integrin Alpha-5 Light Chain |
| Database Links | Reactome: R-HSA-1566948Reactome: R-HSA-1566977Reactome: R-HSA-202733Reactome: R-HSA-216083Reactome: R-HSA-445144Reactome: R-HSA-8941332Reactome: R-HSA-9634597Reactome: R-HSA-9856532Reactome: R-HSA-9860927 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionFocal Adhesion |
| Alternative Antibody Names | Anti-Integrin Alpha-5 antibodyAnti-Cd49 Antigen-Like Family Member E antibodyAnti-Fibronectin Receptor Subunit Alpha antibodyAnti-Integrin Alpha-F antibodyAnti-Vla-5 antibodyAnti-Cd Antigen Cd49e Cleaved Into - Integrin Alpha-5 Heavy Chain - Integrin Alpha-5 Light Chain antibodyAnti-ITGA5 antibodyAnti-FNRA antibody |
Information sourced from Uniprot.org