Anti-IPO8 antibody (878-1037) (STJ116881)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IPO8 (878-1037) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IPO8 |
| Gene ID | 10526 |
| Uniprot ID | IPO8_HUMAN |
| Immunogen Region | 878-1037 |
| Immunogen Sequence | TRQLVNREDRSKAEKADMEE NEEISSDEEETNVTAQAMQS NNGRGEDEEEEDDDWDEEVL EETALEGFSTPLDLDNSVDE YQFFTQALITVQSRDAAWYQ LLMAPLSEDQRTALQEVYTL AEHRRTVAEAKKKIEQQGGF TFENKGVLSAFNFGTVPSNN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 878-1037 of human IPO8 (NP_006381.2). |
Additional Info
| Function | Involved in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, may serve as receptor for nuclear localization signals (NLS) and promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of the signal recognition particle protein SRP19. May also be involved in cytoplasm-to-nucleus shuttling of a broad spectrum of other cargos, including Argonaute-microRNAs complexes, the JUN protein, RELA/NF-kappa-B p65 subunit, the translation initiation factor EIF4E and a set of receptor-activated mothers against decapentaplegic homolog (SMAD) transcription factors that play a critical role downstream of the large family of transforming growth factor beta and bone morphogenetic protein (BMP) cytokines (Probable). |
| Protein Name | Importin-8Imp8Ran-Binding Protein 8Ranbp8 |
| Database Links | Reactome: R-HSA-5578749 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Importin-8 antibodyAnti-Imp8 antibodyAnti-Ran-Binding Protein 8 antibodyAnti-Ranbp8 antibodyAnti-IPO8 antibodyAnti-RANBP8 antibody |
Information sourced from Uniprot.org