Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of various lysates, using BRD7 Rabbit polyclonal antibody (STJ11105077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BRD7 antibody (1-95) (STJ11105077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and BRD7 knockout (KO) 293T cells, using [KO Validated] BRD7 Rabbit polyclonal antibody (STJ22836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-BRD7 antibody (1-95) (STJ22836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-BRD7 antibody (STJA0006799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-BRD7 IP antibody [SAA0193] (STJA0018708)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£378.50 - £1,632.50

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Anti-BRF1 antibody (1-208 aa) (STJ11107523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using TFIIIB90-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-BRF1 antibody (231-280 aa) (STJ95985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using BRF2 Rabbit pAb (STJ116330) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-BRF2 antibody (180-419) (STJ116330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-BRF2 antibody (STJA0008943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using BRIP1 antibody (STJ28887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BRIP1 antibody (1-260) (STJ28887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using BRPF1 Rabbit polyclonal antibody (STJ11102828) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BRPF1 antibody (1-200) (STJ11102828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-BRPF1 antibody (527-577) (STJA0006316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using BTG1 antibody (STJ28442).

Anti-BTG1 antibody (22-171) (STJ28442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from A549 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BTG1 antibody (27-77 aa) (STJ195257)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using BTG2/PC3 Rabbit pAb (STJ111890) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-BTG2 antibody (1-158) (STJ111890)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-BTG2 antibody (STJA0007127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-c-Fos antibody (50-100) (STJ506017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-c-Fos antibody (50-100) {Biotin} (STJ506018)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-c-Fos antibody (50-100) {FITC} (STJ506019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa c-FOS protein.

Anti-c-FOS antibody (Full Length) [2H2] (STJA0003594)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

£454.50

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Western blot analysis of c-fos. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-c-Fos antibody [SM0396] (STJA0010396)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-c-Fos antibody [SM0489] (STJA0010489)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£170.50 - £233.50

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Western blot analysis of lysates from MCF7 cells, using c-Jun Rabbit polyclonal antibody (STJ11102796) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-c-Jun antibody (150-250) (STJ11102796)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin embedded human gastrointestinal stromal tumor using C-kit at dilution of 1:1500

Anti-c-Kit antibody [SM0397] (STJA0010397)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with anti-c-myc antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells.

Anti-c-myc antibody [ZR355] (STJ180501)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£172.50 - £674.50

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Immunofluorescence analysis of NIH/3T3 cells using CAMK2A Rabbit polyclonal antibody (STJ119903) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CAMK2A antibody (300-400) (STJ119903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CAMK2A antibody (STJA0006589)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using CaMKII Rabbit polyclonal antibody (STJ22871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CAMK2B antibody (1-300) (STJ22871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using CaMK2 delta/CAMK2 gamma Rabbit monoclonal antibody (STJ11101232) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CAMK2B antibody (200-300) [S2MR] (STJ11101232)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using CaMKII rabbit monoclonal antibody (STJ11101233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CAMK2B antibody (200-300) [S3MR] (STJ11101233)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-CAMK2B antibody (STJ191965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using CaMKII delta Rabbit mAb (STJ11102452) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-CAMK2D antibody (1-100) [S2MR] (STJ11102452)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from mouse heart, using CaMKII delta Rabbit polyclonal antibody (STJ22872) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-CAMK2D antibody (1-260) (STJ22872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of extracts from K562 cells, using CaMKII Delta Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CAMK2D antibody (Internal) (STJ96428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded Mouse brain using CAMK2G Rabbit polyclonal antibody (STJ11104470) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CAMK2G antibody (300-410) (STJ11104470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot with Preimmune (A) and Anti-CAMK2G Antibody (STJ500361) (B), with peptide on at 1:500 dilution in buffer.

Anti-CAMK2G antibody (400-455) (STJ500361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/DB/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse

£427.50

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Anti-CAMK2G antibody (400-455) {Biotin} (STJ500357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/DB/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CAMK2G antibody (400-455) {FITC} (STJ500358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/DB/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CAMK2G antibody (490-540 aa) (STJ193995)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HepG2 cells, using CaMK4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CAMK4 antibody (166-215 aa) (STJ91992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from rat brain, using CAMK4 Rabbit pAb (STJ119178) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-CAMK4 antibody (304-473) (STJ119178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Immunofluorescence analysis of SH-SY5Y cells using CAMK4 Rabbit polyclonal antibody (STJ27257) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CAMK4 antibody (304-473) (STJ27257)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using CaMKIV monoclonal antibody against Romas, Jurkat cell lysate.

Anti-CAMK4 antibody (STJ98474)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Chicken/Dog/Pig

£144.00 - £348.00

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Western blot detection of CaMKIV in Romas and Jurkat cell lysates using CaMKIV mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:55KDa.

Anti-CAMK4 antibody [3C10-D5-G7] (STJ99119)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various lysates using CAMKIV Rabbit mAb (STJ11103655) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-CAMKIV antibody (374-473) [S5MR] (STJ11103655)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded rat kidney using [KO Validated] PRMT4/CARM1 Rabbit polyclonal antibody (STJ22890) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CARM1 antibody (486-603) (STJ22890)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-CARM1 antibody (STJA0010244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot detection of PRMT4/CARM1 in Hela, A431 and K562 cell lysates using PRMT4/CARM1 mouse mAb (1:200-1:500 diluted).Predicted band size:63KDa.Observed band size:63KDa.

Anti-CARM1 antibody [2B9-H11-H10] (STJ99037)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot analysis using PRMT4 monoclonal antibody against MCF-7 (1) , HeLa (2) , NIH/3T3 (3) , HL-60 (4) , LNcap (5) , Jurkat (6) , PC-3 (7) , Cos7 (8) , and PC-12 (9) cell lysate.

Anti-CARM1 antibody [3H2] (STJ98337)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Rat/Monkey

£144.00 - £348.00

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