• Western blot analysis of various lysates using BAZ2A/ TIP5 Rabbit polyclonal antibody (STJ119688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BAZ2A antibody (97-295) (STJ119688)

SKU:
STJ119688

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BAZ2A/TIP5 (97-295) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAZ2A
Gene ID: 11176
Uniprot ID: BAZ2A_HUMAN
Immunogen Region: 97-295
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 97-295 of human BAZ2A ( TIP5 ) (NP_038477.2).
Immunogen Sequence: QYPSANPGSNLKDPPLLSQF SGGQYPLNGILGGSRQPSSP SHNTNLRAGSQEFWANGTQS PMGLNFDSQELYDSFPDQNF EVMPNGPPSFFTSPQTSPML GSSIQTFAPSQEVGSGIHPD EAAEKEMTSVVAENGTGLVG SLELEEEQPELKMCGYNGSV PSVESLHQEVSVLVPDPTVS CLDDPSHLPDQLEDTPILS
Tissue Specificity Expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Post Translational Modifications Acetylation at Lys-680 by KAT8/MOF promotes its dissociation from pRNA, affecting heterochromatin formation, nucleosome positioning and rDNA silencing. Deacetylation by SIRT1 in late S phase enhances pRNA-binding, allowing de novo DNA methylation and heterochromatin formation. Acetylation is high during S phase and declines to background levels in late S phase when the silent copies of rRNA genes are replicated. Ubiquitinated. Deubiquitinated by USP21 leading to its stabilization.
Function Regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair. Both complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template. Directly stimulates the ATPase activity of SMARCA5 in the NoRC-5 ISWI chromatin remodeling complex. The NoRC-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the NoRC-5 ISWI chromatin remodeling complex. Within the NoRC-5 ISWI chromatin remodeling complex, mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter.pRNA-binding is required for heterochromatin formation and rDNA silencing.
Protein Name Bromodomain Adjacent To Zinc Finger Domain Protein 2a
Transcription Termination Factor I-Interacting Protein 5
Ttf-I-Interacting Protein 5
Tip5
Hwalp3
Database Links Reactome: R-HSA-427413
Cellular Localisation Nucleus
Nucleolus
Colocalizes With The Basal Rna Polymerase I Transcription Factor Ubf In The Nucleolus
Alternative Antibody Names Anti-Bromodomain Adjacent To Zinc Finger Domain Protein 2a antibody
Anti-Transcription Termination Factor I-Interacting Protein 5 antibody
Anti-Ttf-I-Interacting Protein 5 antibody
Anti-Tip5 antibody
Anti-Hwalp3 antibody
Anti-BAZ2A antibody
Anti-KIAA0314 antibody
Anti-TIP5 antibody

Information sourced from Uniprot.org

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