• Western blot analysis of extracts of various cell lines, using WSTF antibody (STJ111893) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using WSTF antibody (STJ111893) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from normal (control) and WSTF knockout (KO) HeLa cells, using WSTF antibody (STJ111893) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using WSTF antibody (STJ111893) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using WSTF antibody (STJ111893) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from normal (control) and WSTF knockout (KO) HeLa cells, using WSTF antibody (STJ111893) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded mouse brain using WSTF antibody (STJ111893) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using WSTF antibody (STJ111893) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-BAZ1B antibody (1-260) (STJ111893)

SKU:
STJ111893

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BAZ1B (1-260) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAZ1B
Gene ID: 9031
Uniprot ID: BAZ1B_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human WSTF (NP_115784.1).
Immunogen Sequence: MAPLLGRKPFPLVKPLPGEE PLFTIPHTQEAFRTREEYEA RLERYSERIWTCKSTGSSQL THKEAWEEEQEVAELLKEEF PAWYEKLVLEMVHHNTASLE KLVDTAWLEIMTKYAVGEEC DFEVGKEKMLKVKIVKIHPL EKVDEEATEKKSDGACDSPS SDKENSSQIAQDHQKKETVV KEDEGRRESINDRARRSPRK LPTSLKKGERKWAPPKFLPH KYDVKLQNEDKIISNVPAD
Tissue Specificity Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.
Function Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Regulatory subunit of the ATP-dependent WICH-1 and WICH-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair. Both complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template. The WICH-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the WICH-5 ISWI chromatin remodeling complex. The WICH-5 ISWI chromatin-remodeling complex regulates the transcription of various genes, has a role in RNA polymerase I transcription. Within the B-WICH complex has a role in RNA polymerase III transcription. Mediates the recruitment of the WICH-5 ISWI chromatin remodeling complex to replication foci during DNA replication.
Protein Name Tyrosine-Protein Kinase Baz1b
Bromodomain Adjacent To Zinc Finger Domain Protein 1b
Williams Syndrome Transcription Factor
Williams-Beuren Syndrome Chromosomal Region 10 Protein
Williams-Beuren Syndrome Chromosomal Region 9 Protein
Hwalp2
Database Links Reactome: R-HSA-5250924
Reactome: R-HSA-5693565
Cellular Localisation Nucleus
Accumulates In Pericentromeric Heterochromatin During Replication
Co-Localizes With Pcna At Replication Foci During S Phase
Co-Localizes With Smarca5/Snf2h At Replication Foci During Late-S Phase
Also Localizes To Replication Foci Independently Of Smarca5/Snf2h And Pcna
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Baz1b antibody
Anti-Bromodomain Adjacent To Zinc Finger Domain Protein 1b antibody
Anti-Williams Syndrome Transcription Factor antibody
Anti-Williams-Beuren Syndrome Chromosomal Region 10 Protein antibody
Anti-Williams-Beuren Syndrome Chromosomal Region 9 Protein antibody
Anti-Hwalp2 antibody
Anti-BAZ1B antibody
Anti-WBSC10 antibody
Anti-WBSCR10 antibody
Anti-WBSCR9 antibody
Anti-WSTF antibody

Information sourced from Uniprot.org

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