![Western blot analysis of various lysates using [KO Validated] WSTF Rabbit pAb (STJ111893) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91989/409014/STJ111893_1__41746.1744895409.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] WSTF Rabbit pAb (STJ111893) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.")
![Western blot analysis of lysates from wild type (WT) and WSTF knockout (KO) HeLa cells, using [KO Validated] WSTF Rabbit pAb (STJ111893) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91989/409015/STJ111893_2__76020.1744895409.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and WSTF knockout (KO) HeLa cells, using [KO Validated] WSTF Rabbit pAb (STJ111893) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
![Immunohistochemistry analysis of paraffin-embedded Rat brain using [KO Validated] WSTF Rabbit pAb (STJ111893) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91989/409016/STJ111893_3__14553.1744895410.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Rat brain using [KO Validated] WSTF Rabbit pAb (STJ111893) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of paraffin-embedded Mouse brain using [KO Validated] WSTF Rabbit pAb (STJ111893) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91989/409017/STJ111893_4__56566.1744895410.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Mouse brain using [KO Validated] WSTF Rabbit pAb (STJ111893) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.")
![Western blot analysis of various lysates using [KO Validated] WSTF Rabbit pAb (STJ111893) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/91989/409014/STJ111893_1__41746.1744895409.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] WSTF Rabbit pAb (STJ111893) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.")
Anti-BAZ1B antibody (1-260) (STJ111893)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal BAZ1B (1-260) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BAZ1B |
| Gene ID | 9031 |
| Uniprot ID | BAZ1B_HUMAN |
| Immunogen Region | 1-260 |
| Immunogen Sequence | MAPLLGRKPFPLVKPLPGEE PLFTIPHTQEAFRTREEYEA RLERYSERIWTCKSTGSSQL THKEAWEEEQEVAELLKEEF PAWYEKLVLEMVHHNTASLE KLVDTAWLEIMTKYAVGEEC DFEVGKEKMLKVKIVKIHPL EKVDEEATEKKSDGACDSPS SDKENSSQIAQDHQKKETVV KEDEGRRESINDRARRSPRK LPTSLKKGERKWAPPKFLPH KYDVKLQNEDKIISNVPAD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human WSTF (NP_115784.1). |
Additional Info
| Tissue Specificity | Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary. |
| Function | Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Regulatory subunit of the ATP-dependent WICH-1 and WICH-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair. Both complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template. The WICH-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the WICH-5 ISWI chromatin remodeling complex. The WICH-5 ISWI chromatin-remodeling complex regulates the transcription of various genes, has a role in RNA polymerase I transcription. Within the B-WICH complex has a role in RNA polymerase III transcription. Mediates the recruitment of the WICH-5 ISWI chromatin remodeling complex to replication foci during DNA replication. |
| Protein Name | Tyrosine-Protein Kinase Baz1bBromodomain Adjacent To Zinc Finger Domain Protein 1bWilliams Syndrome Transcription FactorWilliams-Beuren Syndrome Chromosomal Region 10 ProteinWilliams-Beuren Syndrome Chromosomal Region 9 ProteinHwalp2 |
| Database Links | Reactome: R-HSA-5250924Reactome: R-HSA-5693565 |
| Cellular Localisation | NucleusAccumulates In Pericentromeric Heterochromatin During ReplicationCo-Localizes With Pcna At Replication Foci During S PhaseCo-Localizes With Smarca5/Snf2h At Replication Foci During Late-S PhaseAlso Localizes To Replication Foci Independently Of Smarca5/Snf2h And PcnaLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Baz1b antibodyAnti-Bromodomain Adjacent To Zinc Finger Domain Protein 1b antibodyAnti-Williams Syndrome Transcription Factor antibodyAnti-Williams-Beuren Syndrome Chromosomal Region 10 Protein antibodyAnti-Williams-Beuren Syndrome Chromosomal Region 9 Protein antibodyAnti-Hwalp2 antibodyAnti-BAZ1B antibodyAnti-WBSC10 antibodyAnti-WBSCR10 antibodyAnti-WBSCR9 antibodyAnti-WSTF antibody |
Information sourced from Uniprot.org