• Immunohistochemistry analysis of Bax in paraffin-embedded human colon carcinoma using Bax Rabbit monoclonal antibody (STJ11101764) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Bax in paraffin-embedded rat spleen using Bax Rabbit monoclonal antibody (STJ11101764) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells using Bax Rabbit monoclonal antibody (STJ11101764) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using Bax Rabbit monoclonal antibody (STJ11101764) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using Bax Rabbit monoclonal antibody (STJ11101764) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BAX antibody (1-100) [S4MR] (STJ11101764)

SKU:
STJ11101764

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Bax (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAX
Gene ID: 581
Uniprot ID: BAX_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (Q07812).
Immunogen Sequence: MDGSGEQPRGGGPTSSEQIM KTGALLLQGFIQDRAGRMGG EAPELALDPVPQDASTKKLS ECLKRIGDELDSNMELQRMI AAVDTDSPREVFFRVAADMF
Tissue Specificity Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
Post Translational Modifications Ubiquitinated on Lys-128 and Lys-190. 'Lys-63'-linked polyubiquitin chains on Lys-128 are removed by USP12.
Function Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Protein Name Apoptosis Regulator Bax
Bcl-2-Like Protein 4
Bcl2-L-4
Database Links Reactome: R-HSA-111457
Reactome: R-HSA-114294
Reactome: R-HSA-5620971
Reactome: R-HSA-6803204
Reactome: R-HSA-6804114
Reactome: R-HSA-8878166
Reactome: R-HSA-9603505
Cellular Localisation Isoform Alpha: Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Cytoplasm
Nucleus
Colocalizes With 14-3-3 Proteins In The Cytoplasm
Under Stress Conditions
Undergoes A Conformation Change That Causes Release From Jnk-Phosphorylated 14-3-3 Proteins And Translocation To The Mitochondrion Membrane
Upon Sendai Virus Infection
Recruited To The Mitochondrion Through Interaction With Irf3
Isoform Beta: Cytoplasm
Isoform Gamma: Cytoplasm
Isoform Delta: Cytoplasm
Alternative Antibody Names Anti-Apoptosis Regulator Bax antibody
Anti-Bcl-2-Like Protein 4 antibody
Anti-Bcl2-L-4 antibody
Anti-BAX antibody
Anti-BCL2L4 antibody

Information sourced from Uniprot.org

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