Anti-BARD1 antibody (1-50 aa) (STJ91817)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human BARD1 at the amino acid range 1-50
General Information
| Short Description | Rabbit polyclonal anti-BRCA1-associated RING domain protein 1 (1-50 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BARD1 |
| Gene ID | 580 |
| Uniprot ID | BARD1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human BARD1 at the amino acid range 1-50 |
| Immunogen Region | 1-50 aa |
| Specificity | BARD1 Polyclonal Antibody detects endogenous levels of BARD1 protein. |
Additional Info
| Function | E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage. |
| Protein Name | Brca1-Associated Ring Domain Protein 1Bard-1Ring-Type E3 Ubiquitin Transferase Bard1 |
| Database Links | Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5689603Reactome: R-HSA-5689901Reactome: R-HSA-5693554Reactome: R-HSA-5693565Reactome: R-HSA-5693568Reactome: R-HSA-5693571Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9663199Reactome: R-HSA-9699150Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusDuring S Phase Of The Cell CycleColocalizes With Brca1 Into Discrete Subnuclear FociCan Translocate To The CytoplasmLocalizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs)Recruitment To Dna Damage Sites Is Mediated By The Brca1-A Complex |
| Alternative Antibody Names | Anti-Brca1-Associated Ring Domain Protein 1 antibodyAnti-Bard-1 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Bard1 antibodyAnti-BARD1 antibody |
Information sourced from Uniprot.org