Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NPM1-Thr95 antibody (STJ194733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-NR1D1-Ser55/59 antibody (STJ196400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Glucocorticoid Receptor-S211 Rabbit pAb (STJ116380) at 1:600 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-NR3C1-S211 antibody (STJ116380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of A549 cells using Phospho-GR (S203) Polyclonal Antibody diluted at 1：500

Anti-Phospho-NR3C1-Ser203 antibody (171-220 aa) (STJ90572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-GR (S211) Polyclonal Antibody

Anti-Phospho-NR3C1-Ser211 antibody (181-230 aa) (STJ90761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HELA 293T cells using Phospho-GR (S226) Polyclonal Antibody diluted at 1：500

Anti-Phospho-NR3C1-Ser226 antibody (201-250 aa) (STJ90794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HepG2 cells using Phospho-Nur77 (S351) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-NR4A1-Ser351 antibody (317-366 aa) (STJ91179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-NR4A1-Ser351 antibody (STJ194695)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NR5A1-Ser203 antibody (STJA0009976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of mouse brainrat-brain, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NUAK1-Ser600 antibody (STJA0009944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-Nucleophosmin-Thr199 antibody [R01-2D6] (STJA0035082)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-Phospho-OGT-Thr454 antibody (STJA0009873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-MOR-1 (S375) Polyclonal Antibody

Anti-Phospho-OPRM1-Ser375 antibody (341-390 aa) (STJ91113)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-p27 Kip 1-Ser10 antibody [R05-4F1] (STJA0034670)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MAPK14-T180/Y182 pAb (STJ11107626) at 1:2000 dilution or MAPK14 antibody (STJ228561). HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-p38 MAPK-T180/Y182 antibody (STJ11107626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-p38 MAPK-Y182-Y182 antibody [SRM15123] (STJ11109853)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-p38 MAPK-Y182-Y182 antibody [SRM15147] (STJ11109877)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-p38-Thr180 antibody [R08-4E6] (STJA0034672)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-Phospho-p53-S15-S15 antibody [SRM15140] (STJ11109870)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£0.00

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Anti-Phospho-p53-Ser15 antibody [PT0901R] (STJA0039935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-p53-Ser33 antibody [R04-1B1] (STJA0035772)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-p53-Ser376 antibody [R06-1Y5] (STJA0035935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-p53-Ser392 antibody (STJA0037463)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-p53-Ser392 antibody [PT1223R] (STJA0040171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-p53-Ser392 antibody [R08-2A8] (STJA0035832)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-p53-Ser46 antibody [PT0903R] (STJA0039796)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

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Anti-Phospho-p53-Ser6 antibody [PT1249R] (STJA0040157)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

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Anti-Phospho-p53-Ser6 antibody [R02-0F2] (STJA0035083)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-p53-Ser9 antibody [R03-4Y8] (STJA0035775)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-p53-T18 antibody [23#] (STJA0019168)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Western blot analysis of lysates from C6 cells, using Phospho-p53-T81 Rabbit pAb (STJ11103770) at 1:500 dilution. C6 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-p53-T81 antibody (STJ11103770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Anti-Phospho-p53-Thr55 antibody [R06-4R5] (STJA0035934)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-p95-Ser343 antibody [R04-5S5] (STJA0035850)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-PDK1-Ser-241 antibody (STJA0005522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-PDK1-Ser-241 antibody (STJA0041348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-PDK1-Tyr-9 antibody (STJA0005521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-PDK1-Tyr-9 antibody (STJA0041333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 Rabbit pAb (STJ22329) at 1:1000 dilution. NIH/3T3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-PDPK1-S241 antibody (STJ22329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 pAb (STJ29323) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PDPK1-S241 antibody (STJ29323)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates, using Phospho-PDK1/PDPK1-S241 antibody (STJ11105841) at1:2000 dilution. PC-3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.

Anti-Phospho-PDPK1-S241 antibody [S5841RM] (STJ11105841)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PDK1 (Phospho-Ser241) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PDPK1-Ser241 antibody (210-259 aa) (STJ90382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-PDPK1-Ser241 antibody [PT0869R] (STJA0039151)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using PDK1 (Phospho-Tyr9) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PDPK1-Tyr9 antibody (1-50 aa) (STJ91192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-PR (S190) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PGR-Ser190 antibody (161-210 aa) (STJ90395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser190. The immunolabeling is specifically blocked by the phosphopeptide used as the antigen (not shown).

Anti-Phospho-PGR-Ser190 antibody [1154] (STJA0003755)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human

£488.50

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Western blot analysis of 293 cells using Phospho-PR (S294) Polyclonal Antibody

Anti-Phospho-PGR-Ser294 antibody (261-310 aa) (STJ90691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-PGR-Ser294 antibody [608] (STJA0003756)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Non-Human Primates

£488.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PGR-Ser345 antibody (STJ196391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options