Disease | Research Area | St John's Laboratory

Immunofluorescence analysis of human-heart tissue. 1, PTEN Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PTEN antibody (251-300 aa) (STJ98583)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PTEN (PT2124) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: HEK293 Lane 3: MCF7Predicted band size: 47kDaObserved band size: 47kDa

Anti-PTEN antibody (300-403) [PT2124] (STJA0006236)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PTEN Rabbit monoclonal antibody antibody (STJ11104594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PTEN antibody (304-403) [S4594RM] (STJ11104594)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using P23/PTGES3 Rabbit polyclonal antibody (STJ27278) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PTGES3 antibody (1-100) (STJ27278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PTGES3 antibody (1-100) [S9MR] (STJ11102289)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded rat ovary using P23/PTGES3 Rabbit polyclonal antibody (STJ115361) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PTGES3 antibody (1-110) (STJ115361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using p23 Polyclonal Antibody diluted at 1：2000

Anti-PTGES3 antibody (79-128 aa) (STJ94863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PTK2 antibody at 1:1000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-PTK2 antibody (690aa C-Term) (STJ140154)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Confocal imaging of HeLa cells using FAK Rabbit monoclonal antibody (STJ112977, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-PTK2 antibody (700-800) [S7MR] (STJ112977)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using FAK Rabbit polyclonal antibody (STJ113175) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PTK2 antibody (734-834) (STJ113175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using FAK Rabbit polyclonal antibody (STJ114921) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PTK2 antibody (734-834) (STJ114921)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry of rat tissue with Anti-PTK2 primary antibody (STJ500981) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 5X.

Anti-PTK2 antibody (740-790) (STJ500981)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-PTK2 antibody (740-790) {Biotin} (STJ500982)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-PTK2 antibody (740-790) {FITC} (STJ500983)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of Hela cell. 1, FAK Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-PTK2 antibody (809-858 aa) (STJ93024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using FAK monoclonal antibody against A549 (1) and NIH/3T3 (2) cell lysate.

Anti-PTK2 antibody [10H7] (STJ98061)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot analysis using FAK monoclonal antibody against FAK-hIgGFc transfected HEK293 cell lysate.

Anti-PTK2 antibody [10H7A6] (STJ98062)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-PTK2 antibody [10H7E9] (STJ98063)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot detection of FAK in COS7, HUVEC, A549 and Hela cell lysates using FAK mouse mAb (1:1000 diluted).Predicted band size: 125KDa.Observed band size:125KDa.

Anti-PTK2 antibody [3D8-H11-E12] (STJ99191)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using FAK monoclonal antibody against truncated FAK recombinant protein.

Anti-PTK2 antibody [4A9D6] (STJ98060)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Confocal imaging of NIH/3T3 cells using SHP2 Rabbit monoclonal antibody (STJ11101649, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PTPN11 antibody (1-100) [S9MR] (STJ11101649)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunoprecipitation analysis of 300 Mu g extracts of MCF7 cells using 3 Mu g SHP2 antibody (STJ114360). Western blot was performed from the immunoprecipitate using SHP2 antibody (STJ114360) at a dilution of 1:2000.

Anti-PTPN11 antibody (1-460) (STJ114360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using SH-PTP2 Polyclonal Antibody diluted at 1：2000

Anti-PTPN11 antibody (321-370 aa) (STJ95660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of various cells using SH-PTP2 Polyclonal Antibody diluted at 1：1000

Anti-PTPN11 antibody (508-557 aa) (STJ95659)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 cells using SHP2 Rabbit polyclonal antibody (STJ25224) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-PTPN11 antibody (514-593) (STJ25224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PTPN11 antibody (546-595 aa) (STJ95658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PTPN11 antibody (N-Term) [M163] (STJA0005583)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£319.50

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Anti-PTPN12 antibody (STJ191560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using SHP1 antibody (STJ25228). Blue: DAPI for nuclear staining.

Anti-PTPN6 antibody (100-400) (STJ25228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 3T3 cells using SH-PTP1 Polyclonal Antibody

Anti-PTPN6 antibody (502-551 aa) (STJ95656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using SH-PTP1 Polyclonal Antibody

Anti-PTPN6 antibody (530-579 aa) (STJ95657)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-PTPN6 antibody (C-Term) [M160] (STJA0005580)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot detection of SHP-1 in Human tonsil, Daudi, Jurkat and Raji cell lysates using SHP-1 mouse mAb (1:1000 diluted).Predicted band size:67KDa.Observed band size:67KDa.

Anti-PTPN6 antibody [2B7-H7-F8] (STJ99278)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

£144.00 - £348.00

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Anti-pVHL antibody (150-213 aa) [PT0230] (STJ196795)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of Rat lung using 3 Mu g ASC/TMS1 antibody (STJ119089). Western blot was performed from the immunoprecipitate using ASC/TMS1 antibody (STJ119089) at a dilution of 1:1000.

Anti-PYCARD antibody (1-91) (STJ119089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from 1) K562, 2) Hela, 3) 3T3 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-PYCARD antibody (10-59 aa) (STJ91730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of mouse spleen, using ASC/TMS1 antibody (STJ113135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PYCARD antibody (25-42) (STJ113135)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using ASC/TMS1 Rabbit monoclonal antibody (STJ11105198) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : Jurkat. Exposure time: 180s.

Anti-PYCARD antibody (4-110) [S5198RM] (STJ11105198)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of mouse spleen, using ASC/TMS1 antibody (STJ25245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PYCARD antibody (50-195) (STJ25245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using QKI antibody (STJ11102676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-QK1 antibody (242-341) [S6MR] (STJ11102676)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of MCF7 cells using QKI antibody (STJ29123).

Anti-QKI antibody (1-341) (STJ29123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-QKI antibody (198-248 aa) (STJ195718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of QK1 in 3T3, rat brain lysates using QK1 antibody.

Anti-QKI antibody (285-299 aa) [R01-3F7] (STJA0011861)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Anti-QKI antibody (300-340 aa) (STJ13101706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-QKI antibody (50-100 aa) (STJ13101705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Immunofluorescence analysis of U-2 OS cells using RAB5A Rabbit polyclonal antibody (STJ25263) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RAB5A antibody (1-215) (STJ25263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-Rab5 antibody at 1:1000 dilution tissue lysate at 100 µg per lane Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-RAB5A antibody (115aa C-Term) (STJ140059)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/ELISA

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Anti-Rab5a antibody at 1:500 dilution 293 cells transfected with GFP-Rab5 rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-RAB5A antibody (115aa C-Term) (STJ140060)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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