• Immunohistochemistry analysis of paraffin-embedded mouse pancreas using NOTCH3 antibody (STJ115483) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using NOTCH3 antibody (STJ115483) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of K-562, using NOTCH3 antibody (STJ115483) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NOTCH3 antibody (2305-2321) (STJ115483)

SKU:
STJ115483

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NOTCH3 (2305-2321) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOTCH3
Gene ID: 4854
Uniprot ID: NOTC3_HUMAN
Immunogen Region: 2305-2321
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2305-2321 of human NOTCH3 (NP_000426.2).
Immunogen Sequence: TQLGPQPEVTPKRQVLA
Tissue Specificity Ubiquitously expressed in fetal and adult tissues.
Post Translational Modifications Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. Hydroxylated by HIF1AN.
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Protein Name Neurogenic Locus Notch Homolog Protein 3
Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain
Database Links Reactome: R-HSA-1912399
Reactome: R-HSA-1912408
Reactome: R-HSA-1912420
Reactome: R-HSA-350054
Reactome: R-HSA-5083630
Reactome: R-HSA-9013507
Reactome: R-HSA-9013508
Reactome: R-HSA-9017802
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Notch 3 Intracellular Domain: Nucleus
Following Proteolytical Processing Nicd Is Translocated To The Nucleus
Alternative Antibody Names Anti-Neurogenic Locus Notch Homolog Protein 3 antibody
Anti-Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain antibody
Anti-NOTCH3 antibody

Information sourced from Uniprot.org

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