• Immunofluorescence analysis of HT-29 cells using NOX1 Rabbit polyclonal antibody (STJ114195) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using NOX1 Rabbit polyclonal antibody (STJ114195) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NOX1 Rabbit polyclonal antibody (STJ114195) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NOX1 antibody (200-300) (STJ114195)

SKU:
STJ114195

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NOX1 (200-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOX1
Gene ID: 27035
Uniprot ID: NOX1_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NOX1 (NP_008983.2).
Immunogen Sequence: YFEVFWYTHHLFIFYILGLG IHGIGGIVRGQTEESMNESH PRKCAESFEMWDDRDSHCRR PKFEGHPPESWKWILAPVIL YICERILRFYRSQQKVVITK V
Tissue Specificity NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells.
Function NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
Protein Name Nadph Oxidase 1
Nox-1
Mitogenic Oxidase 1
Mox-1
Nadh/Nadph Mitogenic Oxidase Subunit P65-Mox
Noh-1
Database Links Reactome: R-HSA-5668599
Reactome: R-HSA-9013149
Reactome: R-HSA-9013423
Reactome: R-HSA-9673324
Cellular Localisation Cell Projection
Invadopodium Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Nadph Oxidase 1 antibody
Anti-Nox-1 antibody
Anti-Mitogenic Oxidase 1 antibody
Anti-Mox-1 antibody
Anti-Nadh/Nadph Mitogenic Oxidase Subunit P65-Mox antibody
Anti-Noh-1 antibody
Anti-NOX1 antibody
Anti-MOX1 antibody
Anti-NOH1 antibody

Information sourced from Uniprot.org

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