• Immunohistochemistry analysis of paraffin-embedded rat kidney using NADPH oxidase 4 (NOX4) Rabbit polyclonal antibody (STJ11104654) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using NADPH oxidase 4 (NOX4) Rabbit polyclonal antibody (STJ11104654) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NADPH oxidase 4 (NOX4) antibody (STJ11104654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using NADPH oxidase 4 (NOX4) antibody (STJ11104654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NOX4 antibody (328-578) (STJ11104654)

SKU:
STJ11104654

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nadph Oxidase 4 (328-578) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOX4
Gene ID: 50507
Uniprot ID: NOX4_HUMAN
Immunogen Region: 328-578
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 328-578 of human NADPH oxidase 4 (NOX4) (NP_058627.1).
Immunogen Sequence: KENFKARPGQYITLHCPSVS ALENHPFTLTMCPTETKATF GVHLKIVGDWTERFRDLLLP PSSQDSEILPFIQSRNYPKL YIDGPFGSPFEESLNYEVSL CVAGGIGVTPFASILNTLLD DWKPYKLRRLYFIWVCRDIQ SFRWFADLLCMLHNKFWQEN RPDYVNIQLYLSQTDGIQKI IGEKYHALNSRLFIGRPRWK LLFDEIAKYNRGKTVGVFCC GPNSLSKTLHKLSNQNNSY
Tissue Specificity Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.
Post Translational Modifications Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.
Function Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity. Isoform 4: Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1.
Protein Name Nadph Oxidase 4
Kidney Oxidase-1
Kox-1
Kidney Superoxide-Producing Nadph Oxidase
Renal Nad(Ph-Oxidase
Database Links Reactome: R-HSA-3299685
Reactome: R-HSA-9702518
Reactome: R-HSA-9703465
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Cell Membrane
Cell Junction
Focal Adhesion
May Localize To Plasma Membrane And Focal Adhesions
May Also Localize To The Nucleus
Isoform 4: Nucleus
Nucleus
Nucleolus
Alternative Antibody Names Anti-Nadph Oxidase 4 antibody
Anti-Kidney Oxidase-1 antibody
Anti-Kox-1 antibody
Anti-Kidney Superoxide-Producing Nadph Oxidase antibody
Anti-Renal Nad(Ph-Oxidase antibody
Anti-NOX4 antibody
Anti-RENOX antibody

Information sourced from Uniprot.org

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