Disease | Research Area | St John's Laboratory

Western blot analysis of various lysates using GSDME Rabbit pAb (STJ29568) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GSDME antibody (271-496) (STJ29568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of lysates from 1) Mouse-brain, 2) Hela cells treated by UV 15min, 3) HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody HRP goat anti rabbit (STJ99666) was diluted at 1:10000

Anti-GSDME antibody (400-496) (STJA0010251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-GSDME antibody [R09-7C1] (STJA0034245)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

€166.88 - €351.88

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Anti-GSK-3 beta antibody (341-420aa) [1F7] (STJA0041493)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

€274.38 - €415.63

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Anti-GSK-3 Beta antibody (N-Term) [M131] (STJA0041233)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

€593.13

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Western blot analysis of various lysates using [KO Validated] GSK3 Alpha Rabbit polyclonal antibody (STJ11102944) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GSK3 Alpha antibody (400-483) (STJ11102944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Anti-GSK3 alpha antibody [R07-3A2] (STJA0034393)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€166.88 - €351.88

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Anti-GSK3 alpha/beta antibody [R08-8G7] (STJA0033899)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Hamster

€175.63 - €385.63

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Anti-GSK3 beta antibody [4C4-3H9-10C5] (STJA0032906)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

€166.88 - €351.88

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Anti-GSK3 beta antibody [7D8A6] (STJA0032549)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC

Reactivity :

Human/Mouse/Rat

€166.88 - €351.88

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Anti-GSK3 beta antibody [9B1-5C3-5G8] (STJA0032905)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

€166.88 - €351.88

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Anti-GSK3 beta antibody [9F9-5G6-7G2] (STJA0032904)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

€166.88 - €351.88

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Anti-GSK3 beta antibody [R09-7G8] (STJA0033892)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Hamster

€166.88 - €351.88

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Anti-GSK3 Beta antibody [SRM13034] (STJ11107764)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Anti-GSK3 Beta antibody {ABflo® 647} (STJ11109020)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

€0.00

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g GSK3 Alpha antibody (STJ11101598). Western blot was performed from the immunoprecipitate using GSK3 Alpha antibody (STJ11101598) at a dilution of 1:1000.

Anti-GSK3A antibody (1-100) [S8MR] (STJ11101598)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Western blot analysis of various cells using GSK3 Alpha Polyclonal Antibody

Anti-GSK3A antibody (10-59 aa) (STJ93445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-GSK3α/β (PTR2350) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A549Predicted band size: 52kDaObserved band size: 42kDa

Anti-GSK3A antibody (200-300) [PTR2350] (STJA0006275)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of NIH/3T3 cells using GSK3 Alpha antibody (STJ23877) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-GSK3A antibody (409-483) (STJ23877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Western blot analysis using GSK3 Alpha monoclonal antibody against truncated GSK3 alpha recombinant protein (1) and A549 cell lysate (2).

Anti-GSK3A antibody [6G2F1/6G2F2] (STJ98117)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

€186.25 - €450.00

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Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using GSK3 Beta Mouse mAb diluted at 1:200.

Anti-GSK3B antibody (1-100 aa) [1A6] (STJ97739)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunofluorescence analysis of rat-lung tissue. 1, GSK3 Beta Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-GSK3B antibody (1-50 aa) (STJ93447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Rabbit

€61.25 - €326.25

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Western blot with Anti-GSK3B Antibody (STJ501295) of rat liver at dilution in buffer. Total IgY, B. Affinity purified GSK3b antibody

Anti-GSK3B antibody (1-50) (STJ501295)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€500.63

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Anti-GSK3B antibody (1-50) {Biotin} (STJ501296)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€621.88

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Anti-GSK3B antibody (1-50) {FITC} (STJ501297)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€621.88

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Immunocytochemistry analysis of GSK3 beta (2E6) in HeLa using GSK3 beta antibody.

Anti-GSK3B antibody (313-422 aa) [2E6-D6-C12] (STJA0012948)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

€166.88 - €351.88

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Immunoprecipitation analysis of 200 Mu g extracts of 22Rv1 cells, using 3 Mu g GSK3 Beta antibody (STJ27912). Western blot was performed from the immunoprecipitate using GSK3 Beta antibody (STJ27912) at a dilution of 1:1000.

Anti-GSK3B antibody (320-420) (STJ27912)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of extracts of mouse brain, using GSK3 Beta antibody (STJ23879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GSK3B antibody (350-420) (STJ23879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-GSK3B antibody (N-Term) [M131] (STJA0005433)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

€399.38

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Western blot analysis of extracts from A549, Hela, 3T3 and Rat Brain cell lysates using GSK-3 Beta mouse mAb (1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa.

Anti-GSK3B antibody [2E6-D6-C12] (STJ99095)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse

€186.25 - €450.00

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Western blot analysis using GSK3 Beta monoclonal antibody against A549 (1) , K562 (2) , PC-12 (3) , NIH/3T3 (4) , and HEK293 (5) cell lysate.

Anti-GSK3B antibody [3D10] (STJ98120)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€186.25 - €450.00

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Immunofluorescence analysis of NIH-3T3 cells using Glutathione Synthetase (GSS) (GSS) Rabbit monoclonal antibody (STJ11101358) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GSS antibody (350-450) [S8MR] (STJ11101358)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Western blot analysis of extracts from normal (control) and Glutathione Synthetase (Glutathione Synthetase (GSS) ) knockout (KO) 293T cells, using Glutathione Synthetase (Glutathione Synthetase (GSS) ) antibody (STJ116746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GSS antibody (373-474) (STJ116746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Anti-GSS antibody (STJ193061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-GSS antibody [R02-4A-5] (STJA0033105)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

€268.13 - €385.63

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Western blot analysis of extracts of various cell lines, using GTF2A1 antibody (STJ27298) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-GTF2A1 antibody (1-270) (STJ27298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-GTF2A1 antibody (281-330 aa) (STJ95979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of extracts of NCI-H460 cells, using GTF2A2 antibody (STJ29993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GTF2A2 antibody (1-109) (STJ29993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GTF2A2 antibody (30-110 aa) (STJ190199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of L929 cells using GTF2B Rabbit polyclonal antibody (STJ117782) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GTF2B antibody (40-154) (STJ117782)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis using TFIIB monoclonal antibody against Jurkat, MCF7, HeLa cell lysate.

Anti-GTF2B antibody (STJ98560)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Cow/Chicken/Dog/Pig

€186.25 - €450.00

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Western blot detection of TFIIB in Jurkat, MCF7, COS7, 3T3 and Hela cell lysates using TFIIB mouse mAb (1:500 diluted).Observed band size: 34KDa

Anti-GTF2B antibody [2F6-A3] (STJ99031)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Monkey

€186.25 - €450.00

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Immunofluorescence analysis of U-2 OS cells using GTF2E1 Rabbit polyclonal antibody (STJ117470) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GTF2E1 antibody (230-439) (STJ117470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-GTF2E1 antibody (STJA0008674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of various lysates using GTF2E2 Rabbit pAb (STJ111392) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-GTF2E2 antibody (1-291) (STJ111392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using TFIIE-Beta Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-GTF2E2 antibody (151-200 aa) (STJ95981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of various lysates using GTF2F1 Rabbit polyclonal antibody (STJ11100599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GTF2F1 antibody (390-517) (STJ11100599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GTF2F1 antibody (462-512 aa) (STJ195317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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