![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Glutathione Synthetase (GSS) rabbit polyclonal antibody (STJ116746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95721/377859/STJ116746_IF_2__21941.1713145255.jpg?c=1 "Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Glutathione Synthetase (GSS) rabbit polyclonal antibody (STJ116746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of PC-12 cells using [KO Validated] Glutathione Synthetase (GSS) rabbit polyclonal antibody (STJ116746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95721/377860/STJ116746_IF_3__95048.1713145257.jpg?c=1 "Immunofluorescence analysis of PC-12 cells using [KO Validated] Glutathione Synthetase (GSS) rabbit polyclonal antibody (STJ116746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of U2OS cells using [KO Validated] Glutathione Synthetase (GSS) rabbit polyclonal antibody (STJ116746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95721/377861/STJ116746_IF_4__73741.1713145258.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] Glutathione Synthetase (GSS) rabbit polyclonal antibody (STJ116746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
Anti-GSS antibody (373-474) (STJ116746)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GSS (373-474) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GSS |
| Gene ID | 2937 |
| Uniprot ID | GSHB_HUMAN |
| Immunogen Region | 373-474 |
| Immunogen Sequence | NLYGEEMVQALKQLKDSEER ASYILMEKIEPEPFENCLLR PGSPARVVQCISELGIFGVY VRQEKTLVMNKHVGHLLRTK AIEHADGGVAAGVAVLDNPY PV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human Glutathione Synthetase (Glutathione Synthetase (GSS) ) (NP_000169.1). |
Additional Info
| Function | Catalyzes the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner. Glutathione (gamma-glutamylcysteinylglycine, GSH) is the most abundant intracellular thiol in living aerobic cells and is required for numerous processes including the protection of cells against oxidative damage, amino acid transport, the detoxification of foreign compounds, the maintenance of protein sulfhydryl groups in a reduced state and acts as a cofactor for a number of enzymes. Participates in ophthalmate biosynthesis in hepatocytes. |
| Protein Name | Glutathione SynthetaseGsh SynthetaseGsh-SGlutathione Synthase |
| Database Links | Reactome: R-HSA-174403Reactome: R-HSA-5579006 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Glutathione Synthetase antibodyAnti-Gsh Synthetase antibodyAnti-Gsh-S antibodyAnti-Glutathione Synthase antibodyAnti-GSS antibody |
Information sourced from Uniprot.org