Disease | Research Area | St John's Laboratory

Immunofluorescence analysis of un-treated (left) A549 and UV treated (right) A549 cell. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Anti-GSDMD antibody (1-100) (STJA0010253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit monoclonal antibody (STJ11105210) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. western blot samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Anti-GSDMD antibody (276-484) [S5210RM] (STJ11105210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g GSDMD antibody (STJ11100237). Western blot was performed from the immunoprecipitate using GSDMD antibody (STJ11100237) at a dilution of 1:500.

Anti-GSDMD antibody (346-484) (STJ11100237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U2OS cells using GSDMD Rabbit polyclonal antibody (STJ119438) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GSDMD antibody (346-484) (STJ119438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from THP-1 cells, using GSDMD Rabbit monoclonal antibody (STJ11103428) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-GSDMD antibody (346-484) [S8MR] (STJ11103428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western Blot (WB): Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with STJA0038893

Anti-GSDMD antibody (AA range: 1-100) [PTR1398] (STJA0038893)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using GSDMD (Full Length+N terminal) Rabbit pAb (STJ11106595) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-GSDMD antibody (Full Length + N-Term) (STJ11106595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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GSDMD antibody western blot Jurkat lysates STJA0013051

Anti-GSDMD antibody (Full-length) [R06-5C5] (STJA0013051)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Anti-GSDMD antibody [3D5] (STJ16103516)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human

£746.50

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Anti-GSDMD antibody [6D11] (STJ16103515)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/FC

Reactivity :

Human

£746.50

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Anti-GSDMD antibody [SAA1652] (STJA0020080)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Anti-GSDME antibody (1-100) (STJA0010252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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GSDME antibody western blot U251MG U2OS HepG2 HeLa STJA0011231

Anti-GSDME antibody (1-270 aa) [R07-5I1] (STJA0011231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of extracts from rat stomach, 293 cells, using DFNA5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-GSDME antibody (200-280 aa) (STJ96411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using GSDME Rabbit pAb (STJ29568) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GSDME antibody (271-496) (STJ29568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from 1) Mouse-brain, 2) Hela cells treated by UV 15min, 3) HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody HRP goat anti rabbit (STJ99666) was diluted at 1:10000

Anti-GSDME antibody (400-496) (STJA0010251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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GSDME antibody western blot A549 lysates STJA0034245

Anti-GSDME antibody [R09-7C1] (STJA0034245)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£133.50 - £281.50

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ICC IF of GSK-3 beta in HeLa cells with Alexa Fluor 488 secondary antibody and DAPI nuclear staining

Anti-GSK-3 beta antibody (341-420aa) [1F7] (STJA0041493)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£219.50 - £332.50

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Anti-GSK-3 Beta antibody (N-Term) [M131] (STJA0041233)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£474.50

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Western blot analysis of various lysates using [KO Validated] GSK3 Alpha Rabbit polyclonal antibody (STJ11102944) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GSK3 Alpha antibody (400-483) (STJ11102944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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GSK3 alpha antibody immunocytochemistry 293T cells STJA0034393

Anti-GSK3 alpha antibody [R07-3A2] (STJA0034393)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-GSK3 alpha/beta antibody [R08-8G7] (STJA0033899)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Hamster

£140.50 - £308.50

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GSK3 beta antibody immunohistochemistry human breast carcinoma STJA0032906

Anti-GSK3 beta antibody [4C4-3H9-10C5] (STJA0032906)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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GSK3B antibody flow cytometry NIH/3T3 cells STJA0032549

Anti-GSK3 beta antibody [7D8A6] (STJA0032549)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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GSK3 beta antibody immunohistochemistry human breast carcinoma STJA0032905

Anti-GSK3 beta antibody [9B1-5C3-5G8] (STJA0032905)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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GSK3 beta antibody immunohistochemistry human breast carcinoma STJA0032904

Anti-GSK3 beta antibody [9F9-5G6-7G2] (STJA0032904)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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GSK3 beta antibody western blot Jurkat C6 CHO-K1 HeLa STJA0033892

Anti-GSK3 beta antibody [R09-7G8] (STJA0033892)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Hamster

£133.50 - £281.50

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Anti-GSK3 Beta antibody [SRM13034] (STJ11107764)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Anti-GSK3 Beta antibody {ABflo® 647} (STJ11109020)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

£410.50 - £1,235.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g GSK3 Alpha antibody (STJ11101598). Western blot was performed from the immunoprecipitate using GSK3 Alpha antibody (STJ11101598) at a dilution of 1:1000.

Anti-GSK3A antibody (1-100) [S8MR] (STJ11101598)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various cells using GSK3 Alpha Polyclonal Antibody

Anti-GSK3A antibody (10-59 aa) (STJ93445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-GSK3α/β (PTR2350) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A549Predicted band size: 52kDaObserved band size: 42kDa

Anti-GSK3A antibody (200-300) [PTR2350] (STJA0006275)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 cells using GSK3 Alpha antibody (STJ23877) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-GSK3A antibody (409-483) (STJ23877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis using GSK3 Alpha monoclonal antibody against truncated GSK3 alpha recombinant protein (1) and A549 cell lysate (2).

Anti-GSK3A antibody [6G2F1/6G2F2] (STJ98117)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western Blot (WB): Whole cell lysates of HeLa were separated by 10% SDS-PAGE, and the membrane was blotted with STJ97739

Anti-GSK3B antibody (1-100 aa) [1A6] (STJ97739)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of rat-lung tissue. 1, GSK3 Beta Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-GSK3B antibody (1-50 aa) (STJ93447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Rabbit

£49.00 - £261.00

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Western blot with Anti-GSK3B Antibody (STJ501295) of rat liver at dilution in buffer. Total IgY, B. Affinity purified GSK3b antibody

Anti-GSK3B antibody (1-50) (STJ501295)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-GSK3B antibody (1-50) {Biotin} (STJ501296)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-GSK3B antibody (1-50) {FITC} (STJ501297)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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GSK3 beta antibody western blot A549 HeLa 3T3 rat brain 2E6 STJA0012948

Anti-GSK3B antibody (313-422 aa) [2E6-D6-C12] (STJA0012948)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunoprecipitation analysis of 200 Mu g extracts of 22Rv1 cells, using 3 Mu g GSK3 Beta antibody (STJ27912). Western blot was performed from the immunoprecipitate using GSK3 Beta antibody (STJ27912) at a dilution of 1:1000.

Anti-GSK3B antibody (320-420) (STJ27912)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of mouse brain, using GSK3 Beta antibody (STJ23879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GSK3B antibody (350-420) (STJ23879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Anti-GSK3B antibody (N-Term) [M131] (STJA0005433)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot analysis of extracts from A549, Hela, 3T3 and Rat Brain cell lysates using GSK-3 Beta mouse mAb (1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa.

Anti-GSK3B antibody [2E6-D6-C12] (STJ99095)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse

£149.00 - £360.00

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Western blot analysis using GSK3 Beta monoclonal antibody against A549 (1) , K562 (2) , PC-12 (3) , NIH/3T3 (4) , and HEK293 (5) cell lysate.

Anti-GSK3B antibody [3D10] (STJ98120)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

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Immunofluorescence analysis of NIH-3T3 cells using Glutathione Synthetase (GSS) (GSS) Rabbit monoclonal antibody (STJ11101358) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GSS antibody (350-450) [S8MR] (STJ11101358)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts from normal (control) and Glutathione Synthetase (Glutathione Synthetase (GSS) ) knockout (KO) 293T cells, using Glutathione Synthetase (Glutathione Synthetase (GSS) ) antibody (STJ116746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GSS antibody (373-474) (STJ116746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-GSS antibody (STJ193061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options