Autophagy | Research Area | St John's Laboratory

Western blot analysis of various lysates, using HSP90AA1 Rabbit polyclonal antibody (STJ11105239) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSP90AA1 antibody (433-732 aa) (STJ11105239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Hsp90 Alpha antibody (STJ115462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-HSP90AA1 antibody (433-732) (STJ115462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using Hsp90 Alpha Rabbit polyclonal antibody (STJ24087). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.

Anti-HSP90AA1 antibody (433-732) (STJ24087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of HSP90 in K562, C6, Hela lysates using Hsp90 alpha antibody.

Anti-HSP90AA1 antibody (62-75 aa) [R06-9C1] (STJA0010746)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£264.50

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Western blot analysis of various lysates using Hsp90 Alpha Rabbit pAb (STJ114322) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-HSP90AA1 antibody (629-732) (STJ114322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue with HSP90 a Mouse mAb diluted at 1:2, 000.

Anti-HSP90AA1 antibody (660-740 aa) [1F6] (STJ97496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, HSP90A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HSP90AA1 antibody (683-732 aa) (STJ93622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of Hsp90 in Hela, 3T3, C6, K562 and A549 cell lysates using Hsp90 mouse mAb (1:2000 diluted).Exposion time: 4min.Predicted band size:90KDa.Observed band size:90KDa.

Anti-HSP90AA1 antibody [4B6-G2-H6] (STJ99220)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot detection of Hsp90 alpha in R Brain, Jurkat, CHO-K1, COS7, C6, 3T3 and Hela cell lysates using Hsp90 alpha mouse mAb (1:1000 diluted). Predicted band size:90KDa. Observed band size:90KDa.

Anti-HSP90AA1 antibody [6H7-A3-2G6] (STJ99288)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of U2OS cells using Hsp90 Beta Rabbit polyclonal antibody (STJ11104548). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSP90AB1 antibody (1-300) (STJ11104548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and HSP90AB1 knockout (KO) HeLa cells, using [KO Validated] Hsp90 Beta Rabbit polyclonal antibody (STJ119988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSP90AB1 antibody (1-300) (STJ119988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£129.50 - £642.50

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Immunohistochemistry analysis of Hsp90 Beta in paraffin-embedded Human small intestine tissue using [KO Validated] Hsp90 Beta Rabbit polyclonal antibody (STJ24088) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-HSP90AB1 antibody (1-300) (STJ24088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using HSP90 Beta Polyclonal Antibody diluted at 1：2000

Anti-HSP90AB1 antibody (226-275 aa) (STJ93609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot detection of Hsp90 beta in Hela, 3T3, C6, CHO-K1 and COS7 cell lysates using Hsp90 beta mouse mAb (1:2000 diluted).Exposion time: 4min.Predicted band size:90KDa.Observed band size:90KDa.

Anti-HSP90AB1 antibody [3B9-D4-G4] (STJ99221)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat/Monkey/Hamster

£144.00 - £348.00

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Western blot analysis using HSP90 Beta monoclonal antibody against Jurkat (1) , A431 (2) , HeLa (3) , A549 (4) , HEK293 (5) , K562 (6) , NIH/3T3 (7) , PC-12 (8) and Cos7 (9) cell lysate.

Anti-HSP90AB1 antibody [5G4] (STJ98144)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Western blot analysis of various cells using HSC 70 Polyclonal Antibody diluted at 1：2000

Anti-HSPA8 antibody (202-251 aa) (STJ93596)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using Hsc70/HSPA8 Rabbit polyclonal antibody (STJ24099) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HSPA8 antibody (510-615) (STJ24099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Hsc70/HSPA8 Rabbit monoclonal antibody (STJ11101237) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HSPA8 antibody (547-646) [S7MR] (STJ11101237)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of 1) Hela, 2) HepG2, 3) Raw, 4) Mouse Brain, 5) Rat Brain, 6) Rat Liver using HSC 70 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-HSPA8 antibody (588-638) (STJA0006703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue with HSC70 Mouse mAb diluted at 1:2, 000.

Anti-HSPA8 antibody (Internal) [6C7] (STJ97498)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human BrainTissue using HSC70 mouse Monoclonal antibody diluted at 1:200.

Anti-HSPA8 antibody [7G12] (STJ196659)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of various lysates, using IFT88 antibody (STJ11104895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IFT88 antibody (534-833 aa) [S4895RM] (STJ11104895)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of Rat testis, using IFT88 rabbit polyclonal antibody (STJ115021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IFT88 antibody (534-833) (STJ115021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-IFT88 antibody (STJA0006730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-IFT88 antibody [PT0678R] (STJA0039291)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-K11-Linked Polyubiquitin-Specific antibody [2A3/2E6] (STJA0022260)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Anti-K48-linked Polyubiquitin antibody [SAA0329] (STJA0019256)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat/Rabbit

£378.50 - £1,632.50

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Anti-K63-linked Polyubiquitin antibody [SAA0330] (STJA0019257)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/IF/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of 293T using LAMP2 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-LAMP2 antibody (121-170 aa) (STJ97664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot with Anti-LAMP2 Antibody (STJ501570) of A) LAMP2 positive control, B) Mouse testis.

Anti-LAMP2 antibody (260-310) (STJ501570)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-LAMP2 antibody (260-310) {Biotin} (STJ501571)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-LAMP2 antibody (260-310) {FITC} (STJ501572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of various lysates, using LAMP2 Rabbit mAb (STJ11106864) at1:40000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-LAMP2 antibody (29-375 aa) [SRM] (STJ11106864)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various lysates, using LAMP2 antibody (STJ24369) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-LAMP2 antibody (29-410) (STJ24369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of LAMP2 in paraffin-embedded human liver using LAMP2 Rabbit polyclonal antibody (STJ115952) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-LAMP2 antibody (30-410) (STJ115952)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using LAMP2 Rabbit monoclonal antibody (STJ11101245) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-LAMP2 antibody (311-410) [S5MR] (STJ11101245)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-LAMP2 antibody [PT0249R] (STJA0039664)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£0.00

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Western blot analysis of lysates from wild type (WT) and LAMTOR1 knockout (KO) HeLa cells, using [KO Validated] LAMTOR1 Rabbit polyclonal antibody (STJ11100859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LAMTOR1 antibody (1-161) (STJ11100859)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Confocal immunofluorescence analysis of U2OS cells using LAMTOR1 Rabbit polyclonal antibody (STJ11104413) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-LAMTOR1 antibody (1-161) (STJ11104413)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-LAMTOR2 antibody (10-90 aa) (STJ190366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-LAMTOR3 antibody (1-124 aa) (STJ11107462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-LAMTOR3 antibody (30-110 aa) (STJ191720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-LAMTOR3/MAP2K1IP1 antibody [SAA1946] (STJA0020374)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-LAMTOR4 antibody (8-58 aa) (STJ196554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using LAMTOR5 Rabbit polyclonal antibody (STJ29891) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-LAMTOR5 antibody (1-173) (STJ29891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-LAMTOR5 antibody (1-50 aa) (STJ190865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-LAMTOR5/HBXIP antibody (Met1-Ser91) [1A071] (STJA0021909)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£229.50 - £328.50

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Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using MAP1LC3A antibody (STJ113794) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAP1LC3A antibody (1-100) (STJ113794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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