Anti-FUNDC1 antibody (1-50 aa) (STJ97353)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the N-terminal region of human FUNDC1 at the amino acid range 1-50
General Information
| Short Description | Rabbit polyclonal anti-FUN14 domain-containing protein 1 (1-50 aa) for use in WB, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IF 1:100-300ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FUNDC1 |
| Gene ID | 139341 |
| Uniprot ID | FUND1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human FUNDC1 at the amino acid range 1-50 |
| Immunogen Region | 1-50 aa |
| Specificity | FUNDC1 Polyclonal Antibody detects endogenous levels of FUNDC1 protein. |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-13 by CK2 and at Tyr-18 by SRC inhibits activation of mitophagy. Following hypoxia, dephosphorylated at Tyr-18, leading to interaction with MAP1 LC3 family proteins and triggering mitophagy. Dephosphorylation is mediated by PGAM5. Phosphorylated by ULK1 at Ser-17 which enhances FUNDC1 binding to LC3. Ubiquitinated on Lys-119. Deubiquitinated by USP19.leading to hypoxia-induced DRP1 oligomerization and GTPase activity. |
| Function | Integral mitochondrial outer-membrane protein that mediates the formation of mitochondria-associated endoplasmic reticulum membranes (MAMs). In turn, mediates angiogenesis and neoangiogenesis through interference with intracellular Ca(2+) communication and regulation of the vascular endothelial growth factor receptor KDR/VEGFR2 expression at both mRNA and protein levels. Also acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality and homeostasis, by interacting with and recruiting LC3 protein family to mitochondria. Mechanistically, recruits DRP1 at ER-mitochondria contact sites leading to DRP1 oligomerization and GTPase activity to facilitate mitochondrial fission during hypoxia. Additionally, plays a role in hepatic ferroptosis by interacting directly with glutathione peroxidase/GPX4 to facilitate its recruitment into mitochondria through TOM/TIM complex where it is degraded by mitophagy. |
| Protein Name | Fun14 Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-8934903 |
| Cellular Localisation | Mitochondrion Outer MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Fun14 Domain-Containing Protein 1 antibodyAnti-FUNDC1 antibody |
Information sourced from Uniprot.org