Anti-GABARAP antibody (N-Term) (STJA0005686)

SKU:
STJA0005686-100

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GABARAP (N-Term) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS + 0.02% NaN3
Purification: Antigen Affinity Purified
Dilution Range: WB 1:500-1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GABARAP
Gene ID: 11337
Uniprot ID: GBRAP_HUMAN
Immunogen Region: N-Term
Specificity: Reacts with the N-terminal region of human, mouse and rat GABARAP protein
Immunogen: Synthetic peptide corresponding to amino acids from the N-terminal region of human GABARAP protein
Post Translational Modifications The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, GABARAP-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, GABARAP-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. ATG4B constitutes the major protein for proteolytic activation. ATG4D is the main enzyme for delipidation activity. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS).
Function Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction. Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation. Involved in apoptosis.
Protein Name Gamma-Aminobutyric Acid Receptor-Associated Protein
Gaba(A Receptor-Associated Protein
Mm46
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-8854214
Cellular Localisation Cytoplasmic Vesicle
Autophagosome Membrane
Endomembrane System
Cytoplasm
Cytoskeleton
Golgi Apparatus Membrane
Largely Associated With Intracellular Membrane Structures Including The Golgi Apparatus And Postsynaptic Cisternae
Colocalizes With Microtubules
Localizes Also To Discrete Punctae Along The Ciliary Axoneme
Alternative Antibody Names Anti-Gamma-Aminobutyric Acid Receptor-Associated Protein antibody
Anti-Gaba(A Receptor-Associated Protein antibody
Anti-Mm46 antibody
Anti-GABARAP antibody
Anti-FLC3B antibody
Anti-HT004 antibody

Information sourced from Uniprot.org

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