Phospho Antibodies | St John's Laboratory

Anti-Phospho-BRAF-Ser-446 antibody (STJA0005559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of 293 cells using Phospho-Raf-B (S446) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-BRAF-Ser446 antibody (411-460 aa) (STJ90940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of 3T3 cells using Phospho-Raf-B (S602) Polyclonal Antibody

Anti-Phospho-BRAF-Ser602 antibody (576-625 aa) (STJ90876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from C6 cells, using Phospho-B-Raf-T401 Rabbit mAb (STJ11102615) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-BRAF-T401 antibody [S5MR] (STJ11102615)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using B-RAF (Phospho-Thr599) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BRAF-Thr599 antibody (571-620 aa) (STJ91139)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 30', using B-Raf (Phospho-Thr753) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-BRAF-Thr753 antibody (717-766 aa) (STJ90621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HT-29 cells, using Phospho-BRCA1-S1423 Rabbit pAb (STJ22043) at 1:2000 dilution. HT-29 cells were treated by Anisomycin (5 Mu g/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-BRCA1-S1423 antibody (STJ22043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-BRCA1-S988 Rabbit pAb (STJ22045) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Phospho-BRCA1-S988 antibody (STJ22045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BRCA1 (Phospho-Ser1423) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BRCA1-Ser1423 antibody (1391-1440 aa) (STJ90198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

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Western blot analysis of lysates from 293 cells treated with epo 20U/ml 15', using BRCA1 (Phospho-Ser1457) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-BRCA1-Ser1457 antibody (1423-1472 aa) (STJ90622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western Blot analysis of mouse brain rat brain, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BRCA1-Ser1497 antibody (STJA0010038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BRCA1 (Phospho-Ser1524) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BRCA1-Ser1524 antibody (1491-1540 aa) (STJ90199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-BRCA1-Ser988 antibody (STJA0009869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-BRCA2-Ser3291 antibody (STJ90672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells, using Phospho-BRD4-S1070 Rabbit pAb (STJ11102668) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-BRD4-S1070 antibody (STJ11102668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-BRD4-S1083 Rabbit mAb (STJ11103761) at1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. HeLa cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 300s.

Anti-Phospho-BRD4-S1083 antibody [S1MR] (STJ11103761)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£143.50 - £618.50

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Dot-blot analysis of all sorts of peptides using Phospho-BRD4-S484/S488 antibody (STJ11102664) at 1:1000 dilution.

Anti-Phospho-BRD4-S484/S488 antibody (STJ11102664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Dot-blot analysis of all sorts of peptides using Phospho-BRD4-S492/S494 antibody (STJ11102665) at 1:1000 dilution.

Anti-Phospho-BRD4-S492/S494 antibody (STJ11102665)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-BRD4-S498/S499 Rabbit pAb (STJ11102666) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-BRD4-S498/S499 antibody (STJ11102666)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-BRD4-T204 Rabbit pAb (STJ11101157) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-BRD4-T204 antibody (STJ11101157)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using (STJ11103782) at1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-BRD4-T204 antibody [S2MR] (STJ11103782)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-BRD4-T249 Rabbit polyclonal antibody (STJ11105910) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-BRD4-T249 antibody (STJ11105910)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-BRIP1-Thr1133 antibody (STJ196249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-BRSK2-Thr260 antibody (STJA0009843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of mouse heart, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BTK-Ser179 antibody (STJA0010049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Phospho-BTK-Ser180 antibody (STJ196255)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Btk (Y223) Polyclonal Antibody

Anti-Phospho-BTK-Tyr223 antibody (188-237 aa) (STJ90447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Btk (Y551) Polyclonal Antibody

Anti-Phospho-BTK-Tyr551 antibody (516-565 aa) (STJ90448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Raji cells, using Phospho-BTK-Y223 Rabbit mAb (STJ11102633) at 1:500 dilution. Raji cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-BTK-Y223 antibody [S3MR] (STJ11102633)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Phospho-c-Abl-Tyr-245 antibody (STJA0041063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-c-Abl-Tyr-412 antibody (STJA0041064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-c-Cbl-Tyr-700 antibody [M161] (STJA0041109)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-c-Fos-Ser32 antibody [PT1369R] (STJA0040390)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-c-Jun-Ser63 antibody [PT0608R] (STJA0039219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-c-Myb-Ser11 antibody [PT1200R] (STJA0040193)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-C-Raf-Ser-471 [S578/S433] antibody (STJA0041368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-c-Src-Tyr-215 antibody (STJA0041394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-c-Src-Tyr-530 antibody (C-Term) [M261] (STJA0041379)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using CD88/C5aR (Phospho-Ser338) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-C5AR1-Ser338 antibody (301-350 aa) (STJ91142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-CACNA1C-Ser1981 antibody (STJA0009931)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-CAD-Ser1859 antibody (STJ196260)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using CAD (Phospho-Thr456) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CAD-Thr456 antibody (422-471 aa) (STJ91243)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-CALD1-Ser759 antibody (725-774 aa) (STJ91244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Caldesmon (S789) Polyclonal Antibody

Anti-Phospho-CALD1-Ser789 antibody (744-793 aa) (STJ91034)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Calmodulin (T80/S82) Polyclonal Antibody

Anti-Phospho-Calmodulin-Thr80/S82 antibody (46-95 aa) (STJ91072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CAMK1-Thr177 antibody (143-192 aa) (STJ91073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using Phospho-Camk2a-T286 antibody (STJ11105861) at 1:5000 dilution. Mouse brain cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-Camk2-T286 antibody [S5861RM] (STJ11105861)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-CaMK2-Thr286 antibody (STJ194696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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