• Western blot analysis of lysates from K562 cells treated with starved 24h, using BLNK (Phospho-Tyr84) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using BLNK (Phospho-Tyr84) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BLNK-Tyr84 antibody (50-99 aa) (STJ90962)

SKU:
STJ90962

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-B-cell linker protein-Tyr84 (50-99 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BLNK
Gene ID: 29760
Uniprot ID: BLNK_HUMAN
Immunogen Region: 50-99 aa
Specificity: Phospho-BLNK (Y84) Polyclonal Antibody detects endogenous levels of BLNK protein only when phosphorylated at Y84.
Immunogen: The antiserum was produced against synthesized peptide derived from the human BLNK around the phosphorylation site of Tyr84 at the amino acid range 50-99
Post Translational Modifications Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
Function Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
Protein Name B-Cell Linker Protein
B-Cell Adapter Containing A Sh2 Domain Protein
B-Cell Adapter Containing A Src Homology 2 Domain Protein
Cytoplasmic Adapter Protein
Src Homology 2 Domain-Containing Leukocyte Protein Of 65 Kda
Slp-65
Database Links Reactome: R-HSA-912631
Reactome: R-HSA-9679191
Reactome: R-HSA-983695
Cellular Localisation Cytoplasm
Cell Membrane
Bcr Activation Results In The Translocation To Membrane Fraction
Alternative Antibody Names Anti-B-Cell Linker Protein antibody
Anti-B-Cell Adapter Containing A Sh2 Domain Protein antibody
Anti-B-Cell Adapter Containing A Src Homology 2 Domain Protein antibody
Anti-Cytoplasmic Adapter Protein antibody
Anti-Src Homology 2 Domain-Containing Leukocyte Protein Of 65 Kda antibody
Anti-Slp-65 antibody
Anti-BLNK antibody
Anti-BASH antibody
Anti-SLP65 antibody

Information sourced from Uniprot.org

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