• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using BIK (Phospho-Thr33) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from K562 cells, using BIK (Phospho-Thr33) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-BIK-Thr33 antibody (18-67 aa) (STJ90756)

SKU:
STJ90756

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Bcl-2-interacting killer-Thr33 (18-67 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BIK
Gene ID: 638
Uniprot ID: BIK_HUMAN
Immunogen Region: 18-67 aa
Specificity: Phospho-NBK (T33) Polyclonal Antibody detects endogenous levels of NBK protein only when phosphorylated at T33.
Immunogen: The antiserum was produced against synthesized peptide derived from the human BIK around the phosphorylation site of Thr33 at the amino acid range 18-67
Function Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
Protein Name Bcl-2-Interacting Killer
Apoptosis Inducer Nbk
Bip1
Bp4
Cellular Localisation Endomembrane System
Single-Pass Membrane Protein
Mitochondrion Membrane
Around The Nuclear Envelope
And In Cytoplasmic Membranes
Alternative Antibody Names Anti-Bcl-2-Interacting Killer antibody
Anti-Apoptosis Inducer Nbk antibody
Anti-Bip1 antibody
Anti-Bp4 antibody
Anti-BIK antibody
Anti-NBK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance