Phospho Antibodies | St John's Laboratory

Western blot analysis of various cells using Phospho-NF Kappa B-p65 (S529) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-RELA-Ser529 antibody (496-545 aa) (STJ90350)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of human-lung tissue. 1, NF Kappa B-p65 (phospho Ser529) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-RELA-Ser529 antibody (STJ90349)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Immunofluorescence analysis of Hela cell. 1, NF Kappa B-p65 (phospho Ser536) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-tubulin monoclonal antibody (8F11) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-RELA-Ser536 antibody (502-551 aa) (STJ90351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NF-kB p65/RelA-T434 pAb (STJ11100979) at 1:1000 dilution or RelA antibody (STJ1177811). NIH/3T3 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5min.

Anti-Phospho-RELA-T434 antibody (STJ11100979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of U-2 OS, using Phospho-NF-kB p65/RelA-T505 Rabbit polyclonal antibody (STJ11105817) at 1:1000 dilution. U-2 OS cells were treated by cisplatin at 37 °C for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-RELA-T505 antibody (STJ11105817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Western blot analysis of various cells using Phospho-NF Kappa B-p65 (T254) Polyclonal Antibody

Anti-Phospho-RELA-Thr254 antibody (221-270 aa) (STJ90352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Thr435) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RELA-Thr435 antibody (402-451 aa) (STJ90353)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Thr505) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RELA-Thr505 antibody (471-520 aa) (STJ91119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from A20, using Phospho-RELB-S573 Rabbit polyclonal antibody (STJ11105808) at 1:500 dilution. A20 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-RELB-S573 antibody (STJ11105808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$174.57 - $853.53

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Western blot analysis of lysates from LOVO cells and RAW264.7 cells, using RelB (Phospho-Ser552) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RELB-Ser552 antibody (530-579 aa) (STJ91004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-REPS1-Ser709 antibody (STJ196399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using Ret (Phospho-Tyr1015) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RET-Tyr1015 antibody (991-1040 aa) (STJ90478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis of Ret (phospho Tyr1062) Polyclonal Antibody, using HepG2 cell treated or untreated with forskolin 40nM 30', 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-RET-Tyr1062 antibody (1041-1090 aa) (STJ90679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western Blot analysis of K562 using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-RET-Tyr1096 antibody (STJA0010080)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-Phospho-RET-Tyr905 antibody (881-930 aa) (STJ91308)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from K562 cells, using Phospho-Ret-Y905 Rabbit pAb (STJ29366). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-Ret-Y905 antibody (STJ29366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Western blot analysis of lysates from COS7 cells treated with heat shock, using RGS16 (Phospho-Tyr168) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RGS16-Tyr168 antibody (141-190 aa) (STJ90557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human brain, using Rhodopsin (Phospho-Ser334) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RHO-Ser334 antibody (299-348 aa) (STJ91126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-RHOA-Ser-188 antibody (STJA0005565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$507.15

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Western Blot analysis of mouse kidney, rat kidney. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJ99666 was diluted at 1:10000

Anti-Phospho-RHOA-Ser188 antibody (STJA0009821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RICTOR-Thr1135 antibody (STJ196401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-Phospho-RIP3-S199 antibody [P328] (STJA0019148)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$522.33 - $2,252.85

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Anti-Phospho-RIP3-S227 antibody [P2A11] (STJA0019147)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$522.33 - $2,252.85

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Anti-Phospho-RIP3-T231/S232 antibody [SRM13689] (STJ11108419)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

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Western blot analysis of HT-29 cells, using Phospho-RIPK1-S166 Rabbit monoclonal antibody (STJ11105896) at 1:4000 dilution. HT-29 cells were treated with Z-VAD (50uM) , human TNF-Alpha (50ng/ml) and BV-6 (200nM) at 37 °C for 5 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-RIPK1-S166 antibody [S5896RM] (STJ11105896)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RIPK1-Ser166 antibody (STJ196402)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from HT-29 cells, using Phospho-RIPK1/RIP-S166 Rabbit pAb (STJ11103757) at 1:800 dilution. HT-29 cells were treated with Z-VAD (50uM) , human TNF-Alpha (50ng/ml) and SM-164 (200nM) at 37 °C for 5 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-RIPK1/RIP-S166 antibody (STJ11103757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of 293 cells using Phospho-RIP2 (S176) Polyclonal Antibody

Anti-Phospho-RIPK2-Ser176 antibody (146-195 aa) (STJ90957)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-RIPK2-Ser176 antibody (STJ99600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-RIPK3-S199 antibody [SAA0298] (STJA0019150)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$522.33 - $2,252.85

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Anti-Phospho-RIPK3-S227 antibody [SAA0297] (STJA0019149)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$522.33 - $2,252.85

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Anti-Phospho-RIPK3-Ser227 antibody (STJ196403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human

$67.62 - $360.18

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Western Blot analysis of mouse spleen /Hela, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ROCK2-Tyr722 antibody (STJA0009928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ROR1-Tyr786 antibody (STJA0009911)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western Blot analysis of 1HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ROR2-Ser449 antibody (STJA0009948)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-ROS1-Tyr2114 antibody (STJA0009864)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western Blot analysis of Hela using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-ROS1-Tyr2274 antibody (STJ196404)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from C6 cells, using Phospho-RPA2-S4/S8 Rabbit pAb (STJ11101136) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 45s.

Anti-Phospho-RPA2-S4/S8 antibody (STJ11101136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$174.57 - $853.53

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Western blot analysis of various cells using Phospho-RPA p32 (S33) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-RPA2-Ser33 antibody (1-50 aa) (STJ90636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-RPA2-Ser33 antibody [SRM13700] (STJ11108430)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Mouse/Rat

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-RPA2-Ser8 antibody (STJ194741)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from C6 cells, using Phospho-RPA2-T21 Rabbit mAb (STJ11102614) at 1:1000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-RPA2-T21 antibody [S4MR] (STJ11102614)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$163.53 - $719.67

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Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ug/ml 24h, using RFA2 (Phospho-Thr21) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RPA2-Thr21 antibody (10-59 aa) (STJ90903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-Phospho-Rpb1 CTD-S2/S5-S2/S5 antibody [SRM15121] (STJ11109851)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ChIP/CUT&Tag/ELISA

Reactivity :

Human

$0.00

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Anti-Phospho-Rpb1-Ser2 antibody [PT1003R] (STJA0039928)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$393.30 - $745.20

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Anti-Phospho-Rpb1-Ser5 antibody [PT0923R] (STJA0039821)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Western blot of rat brain lysate showing specific immunolabeling of the ~82 kDa rabphilin 3A phosphorylated at Ser234.

Anti-Phospho-RPH3A-Ser234 antibody (STJA0003761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Rat/Mouse

$627.21

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Immunohistochemistry analysis of paraffin-embedded human brain, using Rabphilin 3A (Phospho-Ser237) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RPH3A-Ser237 antibody (203-252 aa) (STJ91051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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