• Western blot analysis of extracts of Jurkat cells, using Phospho-PLCG1-S1248 antibody (STJ117925) at dilution or PLCG1 antibody (A15704). Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of Phospho-PLC gamma 1 (PLCG1) -S1248 in paraffin-embedded human placenta using Phospho-PLC gamma 1 (PLCG1) -S1248 Rabbit polyclonal antibody (STJ117925) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human placenta using Phospho-PLCG1-S1248 antibody (STJ117925) at dilution of 1:100 (40x lens).
  • Western blot analysis of lysates from Jurkat cells, using Phospho-PLC gamma 1 (PLCG1) -S1248 Rabbit polyclonal antibody (STJ117925) at dilution or PLC gamma 1 (PLCG1) antibody (A15704). Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-PLCG1-S1248 antibody (STJ117925)

SKU:
STJ117925

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PLCG1-S1248 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLCG1
Gene ID: 5335
Uniprot ID: PLCG1_HUMAN
Immunogen: A synthetic phosphorylated peptide around S1248 of human PLC gamma 1 (PLCG1) (NP_002651.2).
Immunogen Sequence: REGSF
Post Translational Modifications Tyrosine phosphorylated in response to signaling via activated FLT3, KIT and PDGFRA. Tyrosine phosphorylated by activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated by activated FLT1 and KDR. Tyrosine phosphorylated by activated PDGFRB. The receptor-mediated activation of PLCG1 involves its phosphorylation by tyrosine kinases, in response to ligation of a variety of growth factor receptors and immune system receptors. For instance, SYK phosphorylates and activates PLCG1 in response to ligation of the B-cell receptor. May be dephosphorylated by PTPRJ. Phosphorylated by ITK and TXK on Tyr-783 upon TCR activation in T-cells. Ubiquitinated by CBLB in activated T-cells.
Function Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Protein Name 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Gamma-1
Plc-148
Phosphoinositide Phospholipase C-Gamma-1
Phospholipase C-Ii
Plc-Ii
Phospholipase C-Gamma-1
Plc-Gamma-1
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-1236382
Reactome: R-HSA-1251932
Reactome: R-HSA-1489509
Reactome: R-HSA-167021
Reactome: R-HSA-1855204
Reactome: R-HSA-186763
Reactome: R-HSA-201556
Reactome: R-HSA-202433
Reactome: R-HSA-2029485
Reactome: R-HSA-210990
Reactome: R-HSA-212718
Reactome: R-HSA-2424491
Reactome: R-HSA-2871796
Reactome: R-HSA-2871809
Reactome: R-HSA-418890
Reactome: R-HSA-5218921
Reactome: R-HSA-5637810
Reactome: R-HSA-5654219
Reactome: R-HSA-5654221
Reactome: R-HSA-5654227
Reactome: R-HSA-5654228
Reactome: R-HSA-5655253
Reactome: R-HSA-5655291
Reactome: R-HSA-5655302
Reactome: R-HSA-5655332
Reactome: R-HSA-8853659
Reactome: R-HSA-9026527
Reactome: R-HSA-9027277
Reactome: R-HSA-9034793
Reactome: R-HSA-9664323
Reactome: R-HSA-9664565
Reactome: R-HSA-9665348
Reactome: R-HSA-9665686
Reactome: R-HSA-9725370
Cellular Localisation Cell Projection
Lamellipodium
Ruffle
Rapidly Redistributed To Ruffles And Lamellipodia Structures In Response To Epidermal Growth Factor (Egf) Treatment
Alternative Antibody Names Anti-1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Gamma-1 antibody
Anti-Plc-148 antibody
Anti-Phosphoinositide Phospholipase C-Gamma-1 antibody
Anti-Phospholipase C-Ii antibody
Anti-Plc-Ii antibody
Anti-Phospholipase C-Gamma-1 antibody
Anti-Plc-Gamma-1 antibody
Anti-PLCG1 antibody
Anti-PLC1 antibody

Information sourced from Uniprot.org

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