Phospho Antibodies | St John's Laboratory

Western blot analysis of lysates from LOVO cells treated with PMA 125ng/ml 30', using PAK5/6 (Phospho-Ser602/Ser560) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PAK5/6-Ser602/S560 antibody (566-615 aa) (STJ90635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-Phospho-PAK6-Ser-165 antibody (STJA0005511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$507.15

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Anti-Phospho-PAK6-Ser-165 antibody (STJA0041338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

$748.65

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Anti-Phospho-pan-PKC ( Beta II Ser660)-Beta II Ser660 antibody [SRM15128] (STJ11109858)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot of mouse whole brain lysate showing specific labeling of the ~86 kDa PanK4 phosphorylated at Ser63 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is nearly eliminated with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-PANK4-Thr63 antibody (STJA0003740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Hamster/Rat

$674.13

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Western blot of HEK293 cells transfected with Parkin wild type (WT) and Parkin S101 mutant (Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser101.

Anti-Phospho-PARK2-Ser101 antibody (STJA0003742)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Non-Human Primates

$674.13

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Western blot of HEK293 cells transfected with Parkin wild type (WT) and Parkin S378 mutant (Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser378.

Anti-Phospho-PARK2-Ser378 antibody (STJA0003743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Non-Human Primates

$674.13

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Anti-Phospho-Parvin alpha-Ser8 antibody [R05-7A7] (STJA0034690)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western Blot analysis of 1 Jurkat cell 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PASK-Thr1165 antibody (STJA0009956)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-Paxillin-Ser-178 antibody (STJA0041327)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-Paxillin-Ser-83 antibody (STJA0041331)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Rat/Mouse

$748.65

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Anti-Phospho-Paxillin-Thr-538 antibody (STJA0041353)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Anti-Phospho-Paxillin-Tyr 118 antibody (STJA0041354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$748.65

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Anti-Phospho-Paxillin-Tyr-31 antibody [M102] (STJA0041307)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$748.65

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Anti-Phospho-Paxillin-Tyr118 antibody [PT0675R] (STJA0039277)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-Phospho-Paxillin-Tyr118 antibody [R04-1V5] (STJA0036157)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of 293T cells using Phospho-PBK (T9) Polyclonal Antibody diluted at 1：500

Anti-Phospho-PBK-Thr9 antibody (1-50 aa) (STJ90490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-Phospho-PBK/TOPK-Thr9 antibody [R21-1A-8] (STJA0035688)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

$184.23 - $388.47

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Western blot analysis of 293 cells using Phospho-Pdcd-4 (S457) Polyclonal Antibody

Anti-Phospho-PDCD4-Ser457 antibody (420-469 aa) (STJ90921)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-PDCD4-Ser67 antibody (33-82 aa) (STJ90547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Anti-Phospho-PDE11A-S163 antibody (100-200) {Biotin} (STJ506174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$761.07

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Anti-Phospho-PDE11A-S163 antibody (100-200) {FITC} (STJ506102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$761.07

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Western Blot analysis of Hela using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-PDE3A-Ser312 antibody (STJA0009951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from K562 cells treated with H2O2 100uM 30', using PDE4D (Phospho-Ser190/53) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PDE4B/C/D-Ser133/119/190 antibody (156-205 aa) (STJ90828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot with Anti-Phospho-PDE4D PKA site (S190) Antibody (STJ502613) with phosphorylated and non-phosphorylated PDE4D3 protein. PDE43 is phosphorylated by catalytic subunit of PKA.

Anti-Phospho-PDE4D PKA site-S190 antibody (50-100) (STJ502613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC-P/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Western Blot analysis of K-562 cell, 2, LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PDE4D-Ser578 antibody (STJA0010001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-PDE6G-Thr22 antibody (STJA0009848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-Phospho-PDGFR beta-Tyr740 antibody [R06-8X3] (STJA0036029)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Mouse/Rat

$184.23 - $388.47

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PDGFRA-Tyr1018 antibody (STJ196378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of PDGFR alpha (Phospho-Tyr754) Antibody. The lane on the right is blocked with the PDGFR alpha (Phospho-Tyr754) peptide.

Anti-Phospho-PDGFRA-Tyr754 antibody (721-770 aa) (STJ91153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PDGFRA-Tyr762 antibody (731-780 aa) (STJ91152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of 293 cells using Phospho-PDGFR-Alpha (Y849) Polyclonal Antibody

Anti-Phospho-PDGFRA-Tyr849 antibody (816-865 aa) (STJ90601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from HepG2 cells, using Phospho-PDGFR alpha-Y1018 Rabbit pAb (STJ29381) at 1:1000 dilution. HepG2 cells were treated by UV for 15-30 minutes or treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PDGFRA-Y1018 antibody (STJ29381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Western blot analysis of lysates from HepG2 cells, using Phospho-PDGFR alpha-Y754 Rabbit pAb (STJ29334) at 1:1000 dilution. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PDGFRA-Y754 antibody (STJ29334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Western blot analysis of lysates from HepG2 cells, using Phospho-PDGFR alpha-Y849 Rabbit pAb (STJ29335) at 1:1000 dilution. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PDGFRA-Y849 antibody (STJ29335)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Anti-Phospho-PDGFRB-Tyr1009 antibody (STJ196379)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-PDGFR-Beta (Y1021) Polyclonal Antibody

Anti-Phospho-PDGFRB-Tyr1021 antibody (991-1040 aa) (STJ91154)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-PDGFRB-Tyr579+Tyr581 antibody (STJA0010057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-PDGFRB-Tyr740 antibody (711-760 aa) (STJ90902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-PDGFRB-Tyr751 antibody (718-767 aa) (STJ91283)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-PDGFRB-Tyr771 antibody (STJ90591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat

$67.62 - $360.18

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Anti-Phospho-PDGFRB-Tyr857 antibody (STJ193215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDGFRB-Y1021 Rabbit pAb (STJ117913) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PDGFRB-Y1021 antibody (STJ117913)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDGFRB-Y740 Rabbit pAb (STJ113494). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PDGFRB-Y740 antibody (STJ113494)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDGFRB-Y751 Rabbit pAb (STJ11100964) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PDGFRB-Y751 antibody (STJ11100964)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of various lysates using Phospho-PDHA1-S293 Rabbit pAb (STJ11103767) at 1:1000 dilution. 293T cells and NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PDHA1-S293 antibody (STJ11103767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb (STJ11102604) at 1:23000 dilution. 293T cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PDHA1-S293 antibody [S4MR] (STJ11102604)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Western blot analysis of extracts of HeLa cells, using Phospho-Tau-T181 antibody (STJ11105820) at1:20000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-PDHA1-S293 antibody [S5820RM] (STJ11105820)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$198.03 - $853.53

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