Western Blot Antibodies | St John's Laboratory

Immunoprecipitation analysis of 300 Mu g extracts from MCF7 cells using 3 Mu g AKT1 Rabbit monoclonal antibody (STJ11101453). Western blot was performed from the immunoprecipitate using AKT1 Rabbit monoclonal antibody (STJ11101453) at a dilution of 1:1000.

Anti-AKT1 antibody (381-480) [S3MR] (STJ11101453)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of extracts from wild type (WT) and AKT1 knockout (KO) HeLa cells, using AKT1 antibody (STJ11103457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AKT1 antibody (381-480) [S7MR] (STJ11103457)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of lysates from 1) 3T3, 2) 293T, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-AKT1 antibody (400-480 aa) (STJ91540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.

Anti-AKT1 antibody (400-480 aa) [1H2] (STJ97728)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

€60.00 - €315.00

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Immunofluorescence analysis of human-lung tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-AKT1 antibody (95-144 aa) (STJ91542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry with Anti-AKT1 Antibody (STJ500060) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-AKT1 antibody (C-Term) (STJ500060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Western blot analysis using Akt1 monoclonal antibody against C2C12, K562 cell lysate.

Anti-AKT1 antibody (C-Term) (STJ98461)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Cow/Chicken/Pig

€180.00 - €435.00

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Anti-AKT1 antibody (C-Term) {Biotin} (STJ500061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-AKT1 antibody (C-Term) {FITC} (STJ500062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€660.63

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STJ73589 (2µg/ml) staining of MCF7 (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence

Anti-AKT1 antibody (Internal) (STJ73589)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Cow

€390.63

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STJ73595 (0. 3µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AKT1 antibody (Internal) (STJ73595)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse

€390.63

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Anti-AKT1 antibody (N-Term) [M101] (STJA0005251)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€399.38

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Western blot detection of AKT1 in 3T3 cell lysates using AKT1 mouse mAb (dilution 1:2000) , with super ECL.Predicted band size:60KDa.Observed band size:60KDa.

Anti-AKT1 antibody [2D9-D2-H3] (STJ99156)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse/Transfected

€180.00 - €435.00

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Western blot analysis using Akt1 monoclonal antibody against NIH/3T3 (1) , HeLa (2) , COS7 (3) and Jurkat (4) cell lysate.

Anti-AKT1 antibody [3A3] (STJ97820)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Monkey

€180.00 - €435.00

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Immunohistochemistry analysis of paraffin-embedded human lung cancer using AKT1/2 Rabbit monoclonal antibody (STJ11103503) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-AKT1/2 antibody (381-480) [S3MR] (STJ11103503)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Western blot analysis of 3T3 cells using Akt1/3 Polyclonal Antibody diluted at 1：1000

Anti-Akt1/3 antibody (406-455 aa) (STJ91551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates, using AKT1/AKT2/AKT3 Rabbit polyclonal antibody (STJ11105345) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-AKT1/AKT2/AKT3 antibody (1-123) (STJ11105345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2/3 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-AKT1/AKT2/AKT3 antibody (281-480 aa) [R04-8A4] (STJA0013034)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat/Hamster

€330.63

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Immunoprecipitation analysis of 25 Mu g extracts of Rat brain cells using 3 Mu g Pan-Akt antibody (STJ11100091). Western blot was performed from the immunoprecipitate using Pan-Akt antibody (STJ11100091) at a dilution of 1:1000.

Anti-AKT1/AKT2/AKT3 antibody (311-480) (STJ11100091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from PC-3 cells using AKT1S1/PRAS40 Rabbit polyclonal antibody (STJ11104478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AKT1S1 antibody (150-256) (STJ11104478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Immunofluorescence analysis of NIH-3T3 cells using AKT1S1/PRAS40 Rabbit polyclonal antibody (STJ27994) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AKT1S1 antibody (150-256) (STJ27994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using AKT1S1/AKT1S1/PRAS40 Rabbit monoclonal antibody (STJ11101861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-AKT1S1 antibody (150-256) [S1MR] (STJ11101861)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€161.88 - €718.13

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Western blot with Anti-AKT1S1 Antibody (STJ502639) of mouse liver. Antibody at 1:500 dilution in buffer.

Anti-AKT1S1 antibody (160-210) (STJ502639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-AKT1S1 antibody (160-210) {Biotin} (STJ502640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-AKT1S1 antibody (160-210) {FITC} (STJ502641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Western blot analysis of various cells using PRAS40 Polyclonal Antibody

Anti-AKT1S1 antibody (207-256 aa) (STJ95215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from 293T cells, using AKT2 Rabbit polyclonal antibody (STJ119992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AKT2 antibody (381-481) (STJ119992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of various cells using Akt2 Polyclonal Antibody diluted at 1：500

Anti-AKT2 antibody (432-481 aa) (STJ91552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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STJ70714 (0. 3µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AKT2 antibody (C-Term) (STJ70714)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog

€390.63

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Western blot analysis using Akt2 monoclonal antibody against A431 (1) , Jurkat (2) , HEK293 (3) , A549 (4) , MCF-7 (5) and PC-12 (6) cell lysate.

Anti-AKT2 antibody [1B6] (STJ97821)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Monkey

€180.00 - €435.00

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Anti-AKT2 antibody [SRM14707] (STJ11109437)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of various lysates using AKT3 Rabbit polyclonal antibody (STJ114775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-AKT3 antibody (90-465) (STJ114775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates, using AKT3 antibody (STJ11104934) at 1:60000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AKT3 antibody (93-154 aa) [S4934RM] (STJ11104934)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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STJ70624 (1µg/ml) staining of HepG2 (A) , Jurkat (B) , Mouse Brain (C) and Rat Brain (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AKT3 antibody (Internal) (STJ70624)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

€390.63

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Western blot analysis of 1) Hela, 2) 3T3, 3) Mouse Brain, 4) Rat Brain tissue, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-AKT3 antibody (STJ197445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis using Akt3 monoclonal antibody against truncated Akt3 recombinant protein (1) and human ovary carcinoma tissue lysate (2).

Anti-AKT3 antibody [3B12F3F10/2D2B11B11/2D2B11H5] (STJ97822)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

€180.00 - €435.00

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Anti-AKTIP antibody (STJA0009274)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using ALAD Rabbit pAb (STJ110696) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ALAD antibody (1-330) (STJ110696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Immunofluorescence analysis of HeLa cells using ALAD antibody (STJ114271) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ALAD antibody (1-330) (STJ114271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using ALAS1 antibody (STJ28604) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-ALAS1 antibody (1-250) (STJ28604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using ALAS1 Rabbit monoclonal antibody (STJ11102160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ALAS1 antibody (541-640) [S0MR] (STJ11102160)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALAS1 antibody (87-137 aa) (STJ193976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-ALAS2 antibody (434-484) (STJA0006585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using ALAS2 Rabbit polyclonal antibody (STJ28605) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALAS2 antibody (50-320) (STJ28605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates, using Albumin Rabbit pAb (STJ22574) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ALB antibody (25-609) (STJ22574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of HepG2 cells using Albumin Rabbit polyclonal antibody (STJ22575) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALB antibody (25-609) (STJ22575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-Albumin antibody at 1:2500 dilution; 10 µl of diluted human serum per lane; Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-ALB antibody (Full Length) (STJ140089)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€124.38 - €466.88

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Western blot analysis using ALB monoclonal antibody against human albumin whole cell lysate.

Anti-ALB antibody (STJ98462)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

€180.00 - €435.00

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