• Immunoprecipitation analysis of 300 Mu g extracts of HT-29 cells using 3 Mu g AHCY antibody (STJ11103506). Western blot was performed from the immunoprecipitate using AHCY antibody (STJ11103506) at a dilution of 1:1000.
  • Western blot analysis of various lysates using AHCY Rabbit monoclonal antibody (STJ11103506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using AHCY Rabbit monoclonal antibody (STJ11103506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AHCY antibody (300-400) [S6MR] (STJ11103506)

SKU:
STJ11103506

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-AHCY (300-400) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AHCY
Gene ID: 191
Uniprot ID: SAHH_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human AHCY (P23526).
Immunogen Sequence: GHFDVEIDVKWLNENAVEKV NIKPQVDRYRLKNGRRIILL AEGRLVNLGCAMGHPSFVMS NSFTNQVMAQIELWTHPDKY PVGVHFLPKKLDEAVAEAHL G
Function Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine. Binds copper ions.
Protein Name Adenosylhomocysteinase
Adohcyase
S-Adenosyl-L-Homocysteine Hydrolase
Database Links Reactome: R-HSA-156581
Reactome: R-HSA-1614635
Reactome: R-HSA-2408508
Reactome: R-HSA-5578997
Cellular Localisation Cytoplasm
Melanosome
Nucleus
Endoplasmic Reticulum
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Adenosylhomocysteinase antibody
Anti-Adohcyase antibody
Anti-S-Adenosyl-L-Homocysteine Hydrolase antibody
Anti-AHCY antibody
Anti-SAHH antibody

Information sourced from Uniprot.org

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