Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from Oryza sativa using plant actin Rabbit pAb (STJ11107562) at1:2000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: (ECL Basic Kit <br>Exposure time: 180s.

Anti-Actin-Plant Specific antibody (1-100 aa) (STJ11107562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

O.sativa

£132.50 - £241.50

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Western blot analysis of lysates from Arabidopsis thaliana tissue using Actin (plant specific) Mouse mAb (STJ11107554). <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-Actin-Plant Specific antibody [SMM5489] (STJ11107554)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Various Plants

£132.50 - £241.50

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Anti-Actin/ACTA1 antibody (13-377aa) [AT2F5] (STJA0041890)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Anti-Actin/ACTA1 antibody [3H5] (STJ16103465)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£746.50

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Anti-Actinin-Alpha 1 antibody [PT1156R] (STJA0040002)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various cells using Actinin-Alpha 1/2/3/4 Polyclonal Antibody diluted at 1：1000

Anti-Actinin-Alpha 1/2/3/4 antibody (21-70 aa) (STJ91466)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Actinin-Alpha 2 antibody [PT0841R] (STJA0039139)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various cells using Actinin-Alpha 2/3 Polyclonal Antibody

Anti-Actinin-Alpha 2/3 antibody (31-80 aa) (STJ91467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Activin A Receptor Type IB antibody [R07-7H9] (STJA0033977)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Activin A Receptor Type IB antibody [R08-7W2] (STJA0037203)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Activin Receptor Type IA antibody [R07-7G4] (STJA0033978)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-Activin Receptor Type IIA antibody [R04-4G9] (STJA0037345)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Activin Receptor Type IIB antibody [R05-7H5] (STJA0037107)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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STJ70505 (2µg/ml) staining of MCF7 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Activin receptor-like kinase 1 antibody (C-Term) (STJ70505)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Immunofluorescence analysis of HUVEC cells, using ACTL6A Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ACTL6A antibody (201-250 aa) (STJ91804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using ACTL6A antibody (STJ27340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ACTL6A antibody (30-120) (STJ27340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ACTL6A antibody [R03-1G4] (STJA0035108)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-ACTL6A antibody [SRM13632] (STJ11108362)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunofluorescence analysis of A-549 cells using ACTL6B antibody (STJ29188).

Anti-ACTL6B antibody (1-280) (STJ29188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ACTL6B antibody (1-80 aa) (STJ190425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-ACTL7A antibody (STJA0007386)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ACTL7B antibody (STJA0008384)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ACTL8 antibody [PT0785R] (STJA0039346)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/IP

Reactivity :

Human

£150.00 - £540.00

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Western blot analysis of lysates from PC-12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ACTL9 antibody (86-136 aa) (STJ193833)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from CACO2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ACTMAP antibody (277-327 aa) (STJ193892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded mouse heart using ACTN1 antibody (STJ22500) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ACTN1 antibody (1-330) (STJ22500)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of 1) 3T3, 2) PC12, 3) Rat Heart Tissue using Actinin-α1 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-ACTN1 antibody (1-80 aa) (STJ91465)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ACTN1 antibody (281-380 aa) [SRM5255] (STJ11107157)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £554.50

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STJ73407 (0. 3µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ACTN1 antibody (596-609) (STJ73407)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Immunohistochemical analysis of paraffin-embedded Human Breast Carrcinoma Tissue using a-actinin Mouse mAb diluted at 1:200.

Anti-ACTN1 antibody [4A12] (STJ97718)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded mouse colon using Sarcomeric alpha Actinin antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-ACTN2 antibody (1-14 aa) [R05-7J8] (STJA0011933)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of HeLa cells using ACTN2 antibody (STJ22501) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ACTN2 antibody (1-250) (STJ22501)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis using Actinin-Alpha 2 monoclonal antibody against HepG2 cell lysate.

Anti-ACTN2 antibody (C-Term) (STJ98460)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Rat/Cow/Chicken/Dog/Pig/Zebrafish

£149.00 - £360.00

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Anti-ACTN2 antibody [SRM13639] (STJ11108369)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various cells using Actinin-Alpha 3 Polyclonal Antibody

Anti-ACTN3 antibody (1-50 aa) (STJ91468)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of ACTN3 in paraffin-embedded rat skeletal muscle using ACTN3 Rabbit polyclonal antibody (STJ114663) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-ACTN3 antibody (580-630) (STJ114663)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of mouse bone marrow cells using ACTN3 Rabbit monoclonal antibody (STJ11103061) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ACTN3 antibody (700-800) [S1MR] (STJ11103061)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-ACTN3 antibody [R09-3H3] (STJA0033979)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g Alpha-Actinin-4 antibody (STJ11101857). Western blot was performed from the immunoprecipitate using Alpha-Actinin-4 antibody (STJ11101857) at a dilution of 1:1000.

Anti-ACTN4 antibody (1-100) [S7MR] (STJ11101857)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunocytochemistry analysis of alpha Actinin 4 (green) in Hela using alpha Actinin 4 antibody, and DAPI (blue)

Anti-ACTN4 antibody (1-14 aa) [R06-3D7] (STJA0010939)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot with Anti-ACTN4 Antibody (STJ500045) of 1) rat podocyte membranes and 2) NIH3TS3 cells. Antibody was diluted 1:500 in buffer.

Anti-ACTN4 antibody (140-190) (STJ500045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£438.50

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Anti-ACTN4 antibody (140-190) {Biotin} (STJ500046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£497.50

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Anti-ACTN4 antibody (140-190) {FITC} (STJ500047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£497.50

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Western blot analysis of various lysates using Alpha-Actinin-4 Rabbit polyclonal antibody (STJ118740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ACTN4 antibody (570-650) (STJ118740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ACTN4 antibody (N-Term) (STJA0005245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£319.50

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Anti-ACTN4 antibody (STJA0006842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using ACTR10 Rabbit polyclonal antibody (STJ118312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ACTR10 antibody (218-417) (STJ118312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ACTR10 antibody (STJA0007293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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