Anti-ACTN1 antibody (281-380 aa) [SRM5255] (STJ11107157)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal ACTN1 (281-380 aa) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5255 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ACTN1 |
| Gene ID | 87 |
| Uniprot ID | ACTN1_HUMAN |
| Immunogen Region | 281-380 aa |
| Immunogen Sequence | KLASDLLEWIRRTIPWLENR VPENTMHAMQQKLEDFRDYR RLHKPPKVQEKCQLEINFNT LQTKLRLSNRPAFMPSEGRM VSDINNAWGCLEQVEKGYEE |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 281-380 of human Alpha-Actinin (NP_001093.1). |
Additional Info
| Function | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation. |
| Protein Name | Alpha-Actinin-1Alpha-Actinin Cytoskeletal IsoformF-Actin Cross-Linking ProteinNon-Muscle Alpha-Actinin-1 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-3000170Reactome: R-HSA-373753Reactome: R-HSA-446388Reactome: R-HSA-9013405Reactome: R-HSA-9013418Reactome: R-HSA-9035034 |
| Cellular Localisation | CytoplasmCytoskeletonMyofibrilSarcomereZ LineCell MembraneCell JunctionCell ProjectionRuffleColocalizes With Myoz2 And Ppp3ca At The Z-Line Of Heart And Skeletal MuscleColocalizes With Psd In Membrane Ruffles And Central Reticular Structures |
| Alternative Antibody Names | Anti-Alpha-Actinin-1 antibodyAnti-Alpha-Actinin Cytoskeletal Isoform antibodyAnti-F-Actin Cross-Linking Protein antibodyAnti-Non-Muscle Alpha-Actinin-1 antibodyAnti-ACTN1 antibody |
Information sourced from Uniprot.org