Anti-ACTN4 antibody (1-100) [S7MR] (STJ11101857)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal alpha Actinin 4 (1-100) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S7MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ACTN4 |
| Gene ID | 81 |
| Uniprot ID | ACTN4_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MVDYHAANQSYQYGPSSAGN GAGGGGSMGDYMAQEDDWDR DLLLDPAWEKQQRKTFTAWC NSHLRKAGTQIENIDEDFRD GLKLMLLLEVISGERLPKPE |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Alpha-Actinin-4 (O43707). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation. |
| Protein Name | Alpha-Actinin-4Non-Muscle Alpha-Actinin 4 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-373753 |
| Cellular Localisation | NucleusCytoplasmCell JunctionCytoskeletonStress FiberPerinuclear RegionLocalized In Cytoplasmic Mrnp Granules Containing Untranslated MrnasExpressed In The Perinuclear Rim And Manchette Structure In Early Elongating Spermatids During SpermiogenesisNuclear Translocation Can Be Induced By The Pi3 Kinase Inhibitor Wortmannin Or By Cytochalasin DExclusively Localized In The Nucleus In A Limited Number Of Cell Lines (Breast Cancer Cell Line Mcf-7Oral Floor Cancer Imc-2And Bladder Cancer Ku-7) |
| Alternative Antibody Names | Anti-Alpha-Actinin-4 antibodyAnti-Non-Muscle Alpha-Actinin 4 antibodyAnti-ACTN4 antibody |
Information sourced from Uniprot.org