• Western blot analysis of extracts of various cell lines, using ACTN2 antibody (STJ22501) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of HeLa cells using ACTN2 antibody (STJ22501) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ACTN2 antibody (1-250) (STJ22501)


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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal antibody anti-ACTN2 (1-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: ACTN2
Gene ID: 88
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ACTN2 (NP_001094.1).
Tissue Specificity Expressed in both skeletal and cardiac muscle.
Post Translational Modifications Ubiquitinated by FBXL22, leading to proteasomal degradation.
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Protein Name Alpha-Actinin-2
Alpha-Actinin Skeletal Muscle Isoform 2
F-Actin Cross-Linking Protein
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-373753
Reactome: R-HSA-390522
Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5673001
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cytoplasm
Z Line
Colocalizes With Myoz1 And Flnc At The Z-Lines Of Skeletal Muscle
Alternative Antibody Names Anti-Alpha-Actinin-2 antibody
Anti-Alpha-Actinin Skeletal Muscle Isoform 2 antibody
Anti-F-Actin Cross-Linking Protein antibody
Anti-ACTN2 antibody

Information sourced from Uniprot.org

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