Anti-ACTN4 antibody (STJA0006842)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human ACTN4
STJA0006842
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General Information

Short DescriptionRabbit polyclonal anti-Alpha-actinin-4 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-2000
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolACTN4
Gene ID81
Uniprot IDACTN4_HUMAN
ImmunogenSynthesized peptide derived from human ACTN4
SpecificityThis antibody detects endogenous levels of ACTN4 at Human, Mouse, Rat

Additional Info

Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation.
Protein Name Alpha-Actinin-4
Non-Muscle Alpha-Actinin 4
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-373753
Cellular Localisation Nucleus
Cytoplasm
Cell Junction
Cytoskeleton
Stress Fiber
Perinuclear Region
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Expressed In The Perinuclear Rim And Manchette Structure In Early Elongating Spermatids During Spermiogenesis
Nuclear Translocation Can Be Induced By The Pi3 Kinase Inhibitor Wortmannin Or By Cytochalasin D
Exclusively Localized In The Nucleus In A Limited Number Of Cell Lines (Breast Cancer Cell Line Mcf-7
Oral Floor Cancer Imc-2
And Bladder Cancer Ku-7)
Alternative Antibody Names Anti-Alpha-Actinin-4 antibody
Anti-Non-Muscle Alpha-Actinin 4 antibody
Anti-ACTN4 antibody

Information sourced from Uniprot.org

Citations

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