Immunohistochemistry Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using AKT1 antibody (STJ27474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AKT1 antibody (100-200) (STJ27474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Anti-Akt1 antibody (251-351 aa) [SRM] (STJ11106868)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

Choose Options

Western blot analysis of extracts of MCF7 cells, using AKT1 antibody (STJ112889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-AKT1 antibody (350-480) (STJ112889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.

Anti-AKT1 antibody (370-477 aa) [10D6] (STJ97724)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat/D.melanogaster

$64.80 - $340.20

Choose Options

Immunofluorescence analysis of PC-12 cells using [KO Validated] AKT1 Rabbit polyclonal antibody (STJ11104319) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AKT1 antibody (380-480) (STJ11104319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Confocal imaging of MCF7 cells using [KO Validated] AKT1 Rabbit monoclonal antibody (STJ11104954, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-AKT1 antibody (380-480) [S4954RM] (STJ11104954)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

Choose Options

Immunoprecipitation analysis of 300 Mu g extracts from MCF7 cells using 3 Mu g AKT1 Rabbit monoclonal antibody (STJ11101453). Western blot was performed from the immunoprecipitate using AKT1 Rabbit monoclonal antibody (STJ11101453) at a dilution of 1:1000.

Anti-AKT1 antibody (381-480) [S3MR] (STJ11101453)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

Choose Options

Western blot analysis of lysates from 1) 3T3, 2) 293T, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-AKT1 antibody (400-480 aa) (STJ91540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Anti-AKT1 antibody (400-480 aa) [1H2] (STJ97728)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

$64.80 - $340.20

Choose Options

Anti-AKT1 antibody (60-140 aa) [1E11] (STJ97771)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

Immunofluorescence analysis of human-lung tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-AKT1 antibody (95-144 aa) (STJ91542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Immunohistochemistry analysis of paraffin-embedded human lung cancer using AKT1/2 Rabbit monoclonal antibody (STJ11103503) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-AKT1/2 antibody (381-480) [S3MR] (STJ11103503)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

Choose Options

Western blot analysis of 3T3 cells using Akt1/3 Polyclonal Antibody diluted at 1：1000

Anti-Akt1/3 antibody (406-455 aa) (STJ91551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2/3 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-AKT1/AKT2/AKT3 antibody (281-480 aa) [R04-8A4] (STJA0013034)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat/Hamster

$357.08

Choose Options

Immunoprecipitation analysis of 25 Mu g extracts of Rat brain cells using 3 Mu g Pan-Akt antibody (STJ11100091). Western blot was performed from the immunoprecipitate using Pan-Akt antibody (STJ11100091) at a dilution of 1:1000.

Anti-AKT1/AKT2/AKT3 antibody (311-480) (STJ11100091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Immunofluorescence analysis of NIH-3T3 cells using AKT1S1/PRAS40 Rabbit polyclonal antibody (STJ27994) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AKT1S1 antibody (150-256) (STJ27994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Western blot with Anti-AKT1S1 Antibody (STJ502639) of mouse liver. Antibody at 1:500 dilution in buffer.

Anti-AKT1S1 antibody (160-210) (STJ502639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$577.13

Choose Options

Anti-AKT1S1 antibody (160-210) {Biotin} (STJ502640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

Choose Options

Anti-AKT1S1 antibody (160-210) {FITC} (STJ502641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

Choose Options

Western blot analysis of various cells using PRAS40 Polyclonal Antibody

Anti-AKT1S1 antibody (207-256 aa) (STJ95215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Immunofluorescence analysis of U2OS cells using Akt2 Rabbit polyclonal antibody (STJ118023) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AKT2 antibody (100-200) (STJ118023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Western blot analysis of various cells using Akt2 Polyclonal Antibody diluted at 1：500

Anti-AKT2 antibody (432-481 aa) (STJ91552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

STJ70714 (0. 3µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AKT2 antibody (C-Term) (STJ70714)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog

$421.88

Choose Options

Western blot analysis using Akt2 monoclonal antibody against A431 (1) , Jurkat (2) , HEK293 (3) , A549 (4) , MCF-7 (5) and PC-12 (6) cell lysate.

Anti-AKT2 antibody [1B6] (STJ97821)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Monkey

$194.40 - $469.80

Choose Options

STJ70624 (1µg/ml) staining of HepG2 (A) , Jurkat (B) , Mouse Brain (C) and Rat Brain (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AKT3 antibody (Internal) (STJ70624)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

$421.88

Choose Options

Immunohistochemistry analysis of paraffin-embedded rat brain using AKTIP antibody (STJ27995) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-AKTIP antibody (1-292) (STJ27995)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Western blot analysis of various lysates, using Albumin Rabbit pAb (STJ22574) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ALB antibody (25-609) (STJ22574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Anti-Albumin antibody [AL-01] (STJ16100600)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/ELISA/WB

Reactivity :

Human

$547.43

Choose Options

Anti-Albumin antibody [AL-01] {Biotin} (STJ16100601)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/ELISA/WB

Reactivity :

Human

$779.63

Choose Options

Anti-Albumin antibody [SM4927] (STJA0004927)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$315.23

Choose Options

Anti-Albumin antibody [SM5071] (STJA0005071)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Rat

$230.18 - $315.23

Choose Options

Immunohistochemistry analysis of paraffin-embedded human ovary carcinoma (A) , kidney carcinoma (B) , lung carcinoma (C) and breast carcinoma (D) , showing cytoplasmic and membrane localization with DAB staining using CD166 monoclonal antibody.

Anti-ALCAM antibody (405-524 aa) [10F1G12] (STJ97908)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

$194.40 - $469.80

Choose Options

STJ70216 (0. 1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Aldehyde Reductase antibody (C-Term) (STJ70216)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

$421.88

Choose Options

Western blot analysis of mouse lung, mouse liver cells using ALDH1A1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ALDH1A1 antibody (21-70 aa) (STJ96522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

Anti-ALDH1A1 antibody (250-350 aa) [SRM] (STJ11106867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

Choose Options

Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using ALDH1A1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-ALDH1A1 antibody (328-343 aa) [R09-3B9] (STJA0010773)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

$357.08

Choose Options

Immunofluorescence analysis of HepG2 cells using ALDH1A1 Rabbit polyclonal antibody (STJ22577) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ALDH1A1 antibody (329-345) (STJ22577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Immunohistochemistry of rat tissue with Anti-ALDH1A1 primary antibody (STJ500063) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-ALDH1A1 antibody (441-491) (STJ500063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Mouse/Rat

$577.13

Choose Options

Anti-ALDH1A1 antibody (441-491) {Biotin} (STJ500064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Mouse/Rat

$713.48

Choose Options

Anti-ALDH1A1 antibody (441-491) {FITC} (STJ500065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Mouse/Rat

$713.48

Choose Options

Formalin-fixed, paraffin-embedded human solitary fibrous tumor stained with anti-ALDH1A1 monoclonal antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic/nuclear staining of tumor cells

Anti-ALDH1A1 antibody (Full length) [ZM71] (STJ180485)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$218.03 - $762.08

Choose Options

STJ70878 (1µg/ml) staining of Human Liver Lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ALDH1A1 antibody (Internal) (STJ70878)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat

$421.88

Choose Options

Western blot analysis using ALDH1A1 monoclonal antibody against Raji (1) , Jurkat (2) , THP-1 (3) and K562 (4) cell lysate.

Anti-ALDH1A1 antibody [5A11] (STJ97824)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$194.40 - $469.80

Choose Options

Western blot analysis of various lysates using ALDH1A2 Rabbit mAb (STJ11102426) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ALDH1A2 antibody (1-100) [S6MR] (STJ11102426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

Choose Options

Immunofluorescence analysis of NIH-3T3 cells using ALDH1A2 antibody (STJ29639) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ALDH1A2 antibody (1-180) (STJ29639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Western blot analysis of lysates from K562 cells, using ALDH1A2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ALDH1A2 antibody (412-461 aa) (STJ91553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Immunofluorescence analysis of U2OS cells using ALDH2 Rabbit polyclonal antibody (STJ22581) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-ALDH2 antibody (321-480) (STJ22581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Western blot analysis of A549 cells using ALDH2 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ALDH2 antibody (41-90 aa) (STJ96720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options