• Immunofluorescence analysis of C6 cells using ADK Rabbit polyclonal antibody (STJ117217) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ADK Rabbit polyclonal antibody (STJ117217) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ADK Rabbit polyclonal antibody (STJ117217) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ADK in paraffin-embedded rat kidney using ADK Rabbit polyclonal antibody (STJ117217) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ADK in paraffin-embedded mouse kidney using ADK Rabbit polyclonal antibody (STJ117217) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ADK in paraffin-embedded human placenta using ADK Rabbit polyclonal antibody (STJ117217) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ADK Rabbit polyclonal antibody (STJ117217) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ADK antibody (200-345) (STJ117217)

SKU:
STJ117217

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADK (200-345) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADK
Gene ID: 132
Uniprot ID: ADK_HUMAN
Immunogen Region: 200-345
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-345 of human ADK (NP_001114.2).
Immunogen Sequence: PFISQFYKESLMKVMPYVDI LFGNETEAATFAREQGFETK DIKEIAKKTQALPKMNSKRQ RIVIFTQGRDDTIMATESEV TAFAVLDQDQKEIIDTNGAG DAFVGGFLSQLVSDKPLTEC IRAGHYAASIIIRRTGCTFP EKPDFH
Tissue Specificity Widely expressed. Highest level in placenta, liver, muscle and kidney.
Function Catalyzes the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Protein Name Adenosine Kinase
Ak
Adenosine 5'-Phosphotransferase
Database Links Reactome: R-HSA-74217
Reactome: R-HSA-9755088
Cellular Localisation Isoform 1: Nucleus
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Adenosine Kinase antibody
Anti-Ak antibody
Anti-Adenosine 5'-Phosphotransferase antibody
Anti-ADK antibody

Information sourced from Uniprot.org

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