Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of various lysates using MAP3K1 Rabbit pAb (STJ118497) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-MAP3K1 antibody (1400-1512) (STJ118497)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MAP3K10 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAP3K10 antibody (391-440) (STJ94150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MLK3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAP3K11 antibody (640-689) (STJ94151)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-MAP3K12 antibody (STJ500390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAP3K12 antibody {Biotin} (STJ500391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAP3K12 antibody {FITC} (STJ500392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of various cells using LZK Polyclonal Antibody

Anti-MAP3K13 antibody (151-200 aa) (STJ93975)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunocytochemistry analysis of MEKK2 (green) in Hela using MEKK2 antibody, and DAPI (blue)

Anti-MAP3K2 antibody (11-27 aa) [R06-2K7] (STJA0011589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis using MEK Kinase-2 monoclonal antibody against HEK293 (1) and MAP3K2-hIgGFc transfected HEK293 (2) cell lysate.

Anti-MAP3K2 antibody [4B4] (STJ98236)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various cells using MEK Kinase-3 Polyclonal Antibody diluted at 1：2000

Anti-MAP3K3 antibody (101-150 aa) (STJ94073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using MAP3K4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAP3K4 antibody (1281-1330 aa) (STJ94074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using ASK 1 Polyclonal Antibody diluted at 1：1000

Anti-MAP3K5 antibody (49-98 aa) (STJ91736)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 293 cells using ASK 1 Polyclonal Antibody

Anti-MAP3K5 antibody (932-981 aa) (STJ91735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using ASK 1 monoclonal antibody against recombinant protein.

Anti-MAP3K5 antibody [2E4] (STJ97852)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MAP3K6 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAP3K6 antibody (281-330 aa) (STJ94075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using TAK1 Rabbit polyclonal antibody (STJ113923) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP3K7 antibody (110-325) (STJ113923)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, Tak1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CK7 monoclonal antibody (12D7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MAP3K7 antibody (161-210 aa) (STJ95901)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of TAK1 in A431 using MAP3K7 antibody.

Anti-MAP3K7 antibody (550-606 aa) [R09-4G4] (STJA0014935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis of TAK1 in Hela, A431 lysates using TAK1 antibody.

Anti-MAP3K7 antibody (593-606 aa) [R09-4B6] (STJA0012028)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£264.50

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Western blot analysis of various cells using COT Polyclonal Antibody

Anti-MAP3K8 antibody (256-305 aa) (STJ92431)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MAP3K9 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAP3K9 antibody (561-610) (STJ94148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MLK1/2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAP3K9/MAP3K10 antibody (281-330) (STJ94149)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells, using MAP4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAP4 antibody (662-711 aa) (STJ94002)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using HPK1 Polyclonal Antibody diluted at 1：1000

Anti-MAP4K1 antibody (371-420 aa) (STJ93590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using HGK Polyclonal Antibody

Anti-MAP4K4 antibody (406-455 aa) (STJ93495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using MAP4K5 antibody (STJ117333) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MAP4K5 antibody (300-480) (STJ117333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 3T3 cells using ERK 2 Polyclonal Antibody diluted at 1：2000

Anti-MAPK1 antibody (136-185 aa) (STJ92992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] ERK2 Rabbit polyclonal antibody (STJ113009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAPK1 antibody (200-300) (STJ113009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (STJ24482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAPK1 antibody (200-300) (STJ24482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis using ERK 2 monoclonal antibody against HeLa (1) , NIH/3T3 (2) , MCF-7 (3) , HEK293 (4) , Jurkat (5) , A549 (6) , NTERA-2 (7) and SMMC-7721 (8) cell lysate.

Anti-MAPK1 antibody [4C11] (STJ98047)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

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Confocal imaging of HeLa cells using ERK1/2 Rabbit monoclonal antibody (STJ11102154, dilution 1:50) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-MAPK1/MAPK3 antibody (180-360) [S4MR] (STJ11102154)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of extracts of various cell lines, using ERK1/ERK2 antibody (STJ119100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAPK1/MAPK3 antibody (200-300) (STJ119100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal immunofluorescence analysis of Hela cells using MAPK1/MAPK3 Rabbit polyclonal antibody (STJ119147) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-MAPK1/MAPK3 antibody (200-300) (STJ119147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using JNK3 monoclonal antibody against NIH/3T3 (1) and SKN-SH (2) cell lysate.

Anti-MAPK10 antibody (28-233 aa) [10E4A4] (STJ98192)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot analysis of various cells using JNK3 Polyclonal Antibody diluted at 1：2000

Anti-MAPK10 antibody (361-410 aa) (STJ93808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 cells using MAPK11 Rabbit polyclonal antibody (STJ110028) at a dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAPK11 antibody (264-364) (STJ110028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, p38 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-MAPK14 antibody (147-196 aa) (STJ94878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Fish/Pig

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of U-87MG cells, using 3 Mu g p38 MAPK antibody (STJ116613). Western blot was performed from the immunoprecipitate using p38 MAPK antibody (STJ116613) at a dilution of 1:1000.

Anti-MAPK14 antibody (261-360) (STJ116613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using ERK 8 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MAPK15 antibody (141-190 aa) (STJ92995)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using MAPK15 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAPK15 antibody (361-410 aa) (STJ92996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using ERK1 antibody (STJ11101698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK3 antibody (1-100) [S8MR] (STJ11101698)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry analysis of ERK1 in paraffin-embedded mouse heart using [KD Validated] ERK1 Rabbit polyclonal antibody (STJ24487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAPK3 antibody (1-378) (STJ24487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using ERK 1 Polyclonal Antibody diluted at 1：500

Anti-MAPK3 antibody (161-210 aa) (STJ92987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 using ERK1 Rabbit monoclonal antibody (STJ11104626) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK3 antibody (200-300) [S4626RM] (STJ11104626)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Rat

£129.50 - £574.50

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Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-MAPK3 antibody (STJ197394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using ERK 1 monoclonal antibody against HeLa (1) , Jurkat (2) , RAW264.7 (3) , HEK293 (4) , K562 (5) , NIH/3T3 (6) , Cos7 (7) and PC-12 (8) cell lysate.

Anti-MAPK3 antibody [1E5] (STJ98045)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of rat-spleen tissue. 1, ERK1 Mouse monoclonal antibody (5E9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-MAPK3 antibody [5E9] (STJ97780)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ70702 (0. 1µg/ml) staining of Human Cerebellum (A) and Frontal Cortex (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-MAPK3/ERK1 antibody (N-Term) (STJ70702)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

£312.50

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