Anti-FZD8 antibody (486-535 aa) (STJ93152)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human FZD8 at the amino acid range 486-535
General Information
| Short Description | Rabbit polyclonal anti-Frizzled-8 (486-535 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FZD8 |
| Gene ID | 8325 |
| Uniprot ID | FZD8_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human FZD8 at the amino acid range 486-535 |
| Immunogen Region | 486-535 aa |
| Specificity | Frizzled-8 Polyclonal Antibody detects endogenous levels of Frizzled-8 protein. |
Additional Info
| Post Translational Modifications | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function | Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1. |
| Protein Name | Frizzled-8Fz-8Hfz8 |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-4608870Reactome: R-HSA-4641263Reactome: R-HSA-5340588 |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinGolgi ApparatusCell MembraneColocalizes With Gopc At The Golgi Apparatus |
| Alternative Antibody Names | Anti-Frizzled-8 antibodyAnti-Fz-8 antibodyAnti-Hfz8 antibodyAnti-FZD8 antibody |
Information sourced from Uniprot.org