• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g FUBP1 antibody (STJ27556). Western blot was performed from the immunoprecipitate using FUBP1 antibody (STJ27556) at a dilution of 1:1000.
  • Immunofluorescence analysis of C6 cells using FUBP1 Rabbit polyclonal antibody (STJ27556) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of FUBP1 in paraffin-embedded mouse brain using FUBP1 Rabbit polyclonal antibody (STJ27556) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FUBP1 in paraffin-embedded human lung cancer using FUBP1 Rabbit polyclonal antibody (STJ27556) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FUBP1 in paraffin-embedded human liver damage using FUBP1 Rabbit polyclonal antibody (STJ27556) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using FUBP1 Rabbit polyclonal antibody (STJ27556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-FUBP1 antibody (302-644) (STJ27556)

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STJ27556

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FUBP1 (302-644) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FUBP1
Gene ID: 8880
Uniprot ID: FUBP1_HUMAN
Immunogen Region: 302-644
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 302-644 of human FUBP1 (NP_003893.2).
Immunogen Sequence: QNDAGVRIQFKPDDGTTPER IAQITGPPDRCQHAAEIITD LLRSVQAGNPGGPGPGGRGR GRGQGNWNMGPPGGLQEFNF IVPTGKTGLIIGKGGETIKS ISQQSGARIELQRNPPPNAD PNMKLFTIRGTPQQIDYARQ LIEEKIGGPVNPLGPPVPHG PHGVPGPHGPPGPPGPGTPM GPYNPAPYNPGPPGPAPHGP PAPYAPQGWGNAYPHWQQQA PPDPAKAGTDPNSAAWAAY
Post Translational Modifications Ubiquitinated. This targets the protein for proteasome-mediated degradation.
Function Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
Protein Name Far Upstream Element-Binding Protein 1
Fbp
Fuse-Binding Protein 1
Dna Helicase V
Hdh V
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Far Upstream Element-Binding Protein 1 antibody
Anti-Fbp antibody
Anti-Fuse-Binding Protein 1 antibody
Anti-Dna Helicase V antibody
Anti-Hdh V antibody
Anti-FUBP1 antibody

Information sourced from Uniprot.org

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