Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of Hela cell. 1, AIM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HSP70 monoclonal antibody (3G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-AIM2 antibody (51-100 aa) (STJ91516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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STJ72169 (1µg/ml) staining of IFNbeta-treated WI-38 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AIM2 antibody (C-Term) (STJ72169)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human

$421.88

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Anti-AIM2 antibody [PT1057R] (STJA0039969)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of Y79 cells using AIPL1 antibody (STJ116048) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AIPL1 antibody (1-384) (STJ116048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of Y79 cells using AIPL1 antibody (STJ28541) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AIPL1 antibody (1-384) (STJ28541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunofluorescence analysis of C6 cells using AIRE Rabbit polyclonal antibody (STJ22559) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AIRE antibody (30-130) (STJ22559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using AIRE-1 Polyclonal Antibody diluted at 1：1000

Anti-AIRE antibody (91-140 aa) (STJ91518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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STJ70203 (1µg/ml) staining of nuclear Jurkat cell (A) , (2ug/ml) Human Spleen (B) and negative control Human Smooth Muscle (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AIRE antibody (C-Term) (STJ70203)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human

$421.88

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Immunofluorescence analysis of HeLa cells using AK1 antibody (STJ22560). Blue: DAPI for nuclear staining.

Anti-AK1 antibody (1-194) (STJ22560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunofluorescence analysis of HepG2 cells, using KAD1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AK1 antibody (101-150 aa) (STJ91521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunocytochemistry analysis of AK2 (green) in Hela using AK2 antibody, and DAPI (blue).

Anti-AK2 antibody (171-184 aa) [R07-4H8] (STJA0012261)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$357.08

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Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g AK4 antibody (STJ22562). Western blot was performed from the immunoprecipitate using AK4 antibody (STJ22562) at a dilution of 1:1000.

Anti-AK4 antibody (1-223) (STJ22562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using AK5 Polyclonal Antibody

Anti-AK5 antibody (391-440 aa) (STJ91522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using AK6 Polyclonal Antibody

Anti-AK6 antibody (11-60 aa) (STJ91523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using AK7 Rabbit polyclonal antibody (STJ116810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-AK7 antibody (1-200) (STJ116810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using AKAP 149 Polyclonal Antibody diluted at 1：2000

Anti-AKAP1 antibody (281-330 aa) (STJ91528)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using AKAP1 Rabbit pAb (STJ11107422). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-AKAP1 antibody (30-280 aa) (STJ11107422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/FC/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of mouse cells using AKAP 10 Polyclonal Antibody

Anti-AKAP10 antibody (10-59 aa) (STJ91524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of HepG2 cells, using AKAP11 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AKAP11 antibody (1761-1810 aa) (STJ91530)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of HeLa cells, using AKAP12 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AKAP12 antibody (301-350 aa) (STJ91531)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using AKAP13 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AKAP13 antibody (721-770 aa) (STJ91526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of Jurkat cells using AKAP 14 Polyclonal Antibody

Anti-AKAP14 antibody (1-50 aa) (STJ91527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of NIH/3T3 cells, using AKAP3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AKAP3 antibody (191-240 aa) (STJ91525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of rat testis cells using AKAP4 Rabbit polyclonal antibody (STJ117013) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-AKAP4 antibody (189-340) (STJ117013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using AKAP 79 Polyclonal Antibody diluted at 1：1000

Anti-AKAP5 antibody (1-50 aa) (STJ91532)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-AKAP6 antibody (30-110 aa) (STJ190212)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using AKAP 95 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-AKAP8 antibody (331-380 aa) (STJ91533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Confocal immunofluorescence analysis of U2OS cells using AKAP8 Rabbit polyclonal antibody (STJ22564) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-AKAP8 antibody (480-692) (STJ22564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-AKAP9 antibody (2610-2690 aa) (STJ190247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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STJ70660 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-AKAP9/AKAP450/CG-NAP antibody (C-Term) (STJ70660)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse

$421.88

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Western blot analysis of various cells using BCA3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-AKIP1 antibody (111-160 aa) (STJ91827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of HeLa cells using AKR1A1 antibody (STJ115538).

Anti-AKR1A1 antibody (1-325) (STJ115538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of PC-12 cells using AKR1B1 Rabbit polyclonal antibody (STJ22567) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-AKR1B1 antibody (1-316) (STJ22567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using Aldose Reductase Polyclonal Antibody

Anti-AKR1B1 antibody (241-290 aa) (STJ91559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-AKR1C1 antibody (30-110 aa) (STJ92669)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of U-2 OS cells using AKR1C2 Polyclonal Antibody (STJ116408) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AKR1C2 antibody (1-323) (STJ116408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of COLO cells using DD2 Polyclonal Antibody

Anti-AKR1C2 antibody (21-70 aa) (STJ92670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunoprecipitation analysis of 200 Mu g extracts of K-562 cells, using 3 Mu g AKR1C3 antibody (STJ22568). Western blot was performed from the immunoprecipitate using AKR1C3 antibody (STJ22568) at a dilution of 1:1000.

Anti-AKR1C3 antibody (1-323) (STJ22568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Confocal imaging of A549 cells using AKR1C3 Rabbit monoclonal antibody (STJ11101962, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-AKR1C3 antibody (200-300) [S2MR] (STJ11101962)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

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Immunofluorescence analysis of A549 cells, using AKR1CL1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AKR1C8 antibody (80-129 aa) (STJ91535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using AKR1CL2 Polyclonal Antibody diluted at 1：500

Anti-AKR1E2 antibody (141-190 aa) (STJ91536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of human-stomach tissue. 1, Akt Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Akt antibody (281-330 aa) (STJ91550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using AKT1 antibody (STJ27474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AKT1 antibody (100-200) (STJ27474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of MCF7 cells, using AKT1 antibody (STJ112889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-AKT1 antibody (350-480) (STJ112889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.

Anti-AKT1 antibody (370-477 aa) [10D6] (STJ97724)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat/D.melanogaster

$64.80 - $340.20

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Immunofluorescence analysis of PC-12 cells using [KO Validated] AKT1 Rabbit polyclonal antibody (STJ11104319) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AKT1 antibody (380-480) (STJ11104319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Confocal imaging of MCF7 cells using [KO Validated] AKT1 Rabbit monoclonal antibody (STJ11104954, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-AKT1 antibody (380-480) [S4954RM] (STJ11104954)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of lysates from 1) 3T3, 2) 293T, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-AKT1 antibody (400-480 aa) (STJ91540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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