Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of human-liver-cancer tissue. 1, FAS Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-FAS antibody (257-306 aa) (STJ93041)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse/Dog

$64.80 - $340.20

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Western blot analysis of various cells using CD95 Polyclonal Antibody diluted at 1：500

Anti-FAS antibody (281-330 aa) (STJ92150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-Fas antibody [PT0128R] (STJA0039648)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

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Immunohistochemistry analysis of paraffin-embedded human gallbladder using FAS at dilution of 1:200

Anti-Fas antibody [SM0400] (STJA0010400)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse

$230.18 - $315.23

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Anti-Fascin antibody (250-350) [ABT-FASN] (STJA0039000)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Fascin antibody [PT0620R] (STJA0039141)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of Fascin at dilution of 1:1000

Anti-Fascin antibody [SM0413] (STJA0010413)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

$230.18 - $315.23

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Anti-Fascin antibody [SM5027] (STJA0005027)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$315.23

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Western blot analysis of lysates from 1) HepG2, 2) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5G3) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-FASLG antibody (101-150 aa) (STJ93042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Pig

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded Human-colon-cancer, antibody was diluted at 1:100

Anti-FASLG antibody (121-170 aa) (STJ99000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using Fatty Acid Synthase Polyclonal Antibody diluted at 1：1000

Anti-FASN antibody (1478-1527 aa) (STJ93048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using Fatty Acid Synthase (FASN) antibody (STJ23631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FASN antibody (2221-2511) (STJ23631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Fatty Acid Synthase (FASN) antibody (STJ11101588). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase (FASN) antibody (STJ11101588) at a dilution of 1:500.

Anti-FASN antibody (2400-2500) [S8MR] (STJ11101588)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot detection of Fatty Acid Synthase in Hela, C6, 3T3, CHO-K1 and 293 cell lysates using Fatty Acid Synthase mouse mAb (dilution 1:1000).Predicted band size:273kDa.Observed band size:273kDa.

Anti-FASN antibody [2F9-B2-B6] (STJ99046)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Human/Mouse/Rat/Monkey/Cow

$194.40 - $469.80

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Western blot analysis of various cells using FASTKD2 Polyclonal Antibody

Anti-FASTKD2 antibody (171-220 aa) (STJ93046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using FASTKD3 Polyclonal Antibody

Anti-FASTKD3 antibody (121-170 aa) (STJ93047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using FASTKD5 Rabbit polyclonal antibody (STJ119787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FASTKD5 antibody (495-764) (STJ119787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-FAT1 antibody (1140-1220 aa) (STJ190301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-FAT3 antibody (896-946 aa) (STJ196598)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from U-87MG cells, using FAT4 Rabbit pAb (STJ11102882) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-FAT4 antibody (4900-4981) (STJ11102882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-Fatty Acid Synthase antibody (2212-2511 aa) [SRM] (STJ11106857)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-Fatty Acid Synthase antibody [PT0554R] (STJA0039439)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of FASN at dilution of 1:1000

Anti-Fatty Acid Synthase antibody [SM0497] (STJA0010497)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

$230.18 - $315.23

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 1 Mu g Fibrillarin/U3 RNP antibody (STJ115451). Western blot was performed from the immunoprecipitate using Fibrillarin/U3 RNP antibody (STJ115451) at a dilution of 1:1000.

Anti-FBL antibody (100-321) (STJ115451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$151.88 - $708.08

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Confocal imaging of U-2OS cells using Fibrillarin/U3 RNP Rabbit monoclonal antibody (STJ11101258, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-FBL antibody (222-321) [S8MR] (STJ11101258)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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STJ70401 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-FBL2/FBXL2 antibody (N-Term) (STJ70401)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IF

Reactivity :

Human/Rat/Dog/Cow

$421.88

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Western blot analysis of various cells using Fibulin-2 Polyclonal Antibody diluted at 1：500

Anti-FBLN2 antibody (241-290 aa) (STJ93072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using FBLN5 Rabbit pAb (STJ112002) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-FBLN5 antibody (199-448) (STJ112002)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis using Fibulin-5 monoclonal antibody against truncated fibulin5 recombinant protein (1) and HeLa cell lysate (2).

Anti-FBLN5 antibody [3F10A5/3F8A12] (STJ98074)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis of mouse-liver cells using FBN1 Polyclonal Antibody

Anti-FBN1 antibody (2811-2860 aa) (STJ93049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of Rat pancreas, using Fibrillin 1 antibody (STJ119094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FBN1 antibody (360-460) (STJ119094)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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STJ72053 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-FBN1 antibody (Internal) (STJ72053)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

$421.88

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Anti-FBN3 antibody (1311-1361 aa) (STJ196573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of mouse kidney using FBP1 Rabbit monoclonal antibody (STJ11101382) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-FBP1 antibody (239-338) [S2MR] (STJ11101382)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunofluorescence analysis of U-2 OS cells using FBP1 Rabbit polyclonal antibody (STJ27359) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-FBP1 antibody (50-150) (STJ27359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using FBP2 Rabbit pAb (STJ111892) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-FBP2 antibody (1-339) (STJ111892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Anti-FBX32 antibody [PT0970R] (STJA0039773)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of L929 cells using FBXO21 Rabbit polyclonal antibody (STJ118547) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-FBXO21 antibody (440-621) (STJ118547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of Mouse leg bone tissue using FBXO32 Rabbit pAb (STJ11107064) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining.

Anti-FBXO32 antibody (201-300 aa) (STJ11107064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of A431 cells using Fbx32/FBOX32 rabbit polyclonal antibody (STJ113795) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-FBXO32 antibody (206-355) (STJ113795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of mouse skeletal muscle, using Fbx32/FBXO32 antibody (STJ23637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-FBXO32 antibody (206-355) (STJ23637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle using Fbx32/FBXO32 Rabbit polyclonal antibody (STJ28908) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-FBXO32 antibody (206-355) (STJ28908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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STJ70410 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-FBXO44 antibody (C-Term) (STJ70410)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

$421.88

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Western blot analysis of various lysates using FBXO6 Rabbit pAb (STJ118010) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-FBXO6 antibody (70-260) (STJ118010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunofluorescence analysis of A549 cells using FBXO7 antibody (STJ29600).

Anti-FBXO7 antibody (353-522) (STJ29600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of A-431 cells, using FBXW11 antibody (STJ110094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FBXW11 antibody (519-542) (STJ110094)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using FBXW11 Rabbit pAb (STJ11106589) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-FBXW11 antibody (STJ11106589)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-FBXW7 antibody [PT1440R] (STJA0040302)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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