Immunocytochemistry Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (STJ27585) at 1:500 dilution. 293T cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. C6 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-MAP1LC3A/MAP1LC3B antibody (1-60) (STJ27585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry analysis of LC3B in paraffin-embedded mouse brain using LC3B Rabbit polyclonal antibody (STJ11103828) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAP1LC3B antibody (1-100) (STJ11103828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of paraffin-embedded rat brain using [KD Validated] LC3B Rabbit polyclonal antibody (STJ29278) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP1LC3B antibody (1-100) (STJ29278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of 293T cells, using LC3B antibody (STJ113056) at 1:1000 dilution. 293T cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-MAP1LC3B antibody (1-125) (STJ113056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunofluorescence analysis of 293T and 293T Chloroquine cells using LC3B Rabbit polyclonal antibody (STJ113754) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP1LC3B antibody (1-125) (STJ113754)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-MAP1LC3B antibody (2-107) [S4MR] (STJ11101754)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of extracts of mouse brain, using MAP2 rabbit polyclonal antibody antibody (STJ114887) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-MAP2 antibody (1-118) (STJ114887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Anti-Map2 antibody (1510-1827) (STJ119531)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of mouse brain cells using MAP2 Rabbit polyclonal antibody (STJ24462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Map2 antibody (1510-1827) (STJ24462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~70 kDa MAP2C/D proteins and the ~280 kDa MAP2A/B proteins.

Anti-MAP2 antibody (Full Length) [2C4] (STJA0003677)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

$613.58

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Western blot detection of MAP2 (N-terminus) in Mouse Brain tissue and Hela cell lysates using MAP2 (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 202KDa.Observed band size: 300KDa.

Anti-MAP2 antibody (N-Term) [1D7-B9-C8-F6] (STJ99309)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Mouse

$194.40 - $469.80

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Immunofluorescence of a section of rat brain stem showing colabeled with Anti-MAP2 (STJA0003676, red, 1:5000) and Anti-FOX3 ( cat. STJA0003841, green, 1:1000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.

Anti-MAP2 antibody (STJA0003676)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

$544.73

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Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.

Anti-MAP2-MAP2 antibody (STJA0003848)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat

$613.58

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Western blot of mouse cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.

Anti-MAP2-MAP2 antibody [4H5] (STJA0003847)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

$613.58

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Immunofluorescence analysis of U2OS cells using MEK1 Rabbit polyclonal antibody (STJ24463). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP2K1 antibody (1-100) (STJ24463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain using 3 Mu g MEK1 antibody (STJ11101700). Western blot was performed from the immunoprecipitate using MEK1 (STJ11101700) at a dilution of 1:1000.

Anti-MAP2K1 antibody (1-100) [S0MR] (STJ11101700)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of MEK1 in (1) A431 lysates; (2) HeLa lysates using MEK1 antibody.

Anti-MAP2K1 antibody (1-40 aa) [R07-8C5] (STJA0015763)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat/Cow/Dog

$357.08

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Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit polyclonal antibody (STJ116970) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MAP2K2 antibody (1-100) (STJ116970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunofluorescence analysis of U-2 OS cells using MEK3 antibody (STJ110029) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MAP2K3 antibody (1-70) (STJ110029)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of MCF7 cells using MEK5 Rabbit polyclonal antibody (STJ29033). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP2K5 antibody (1-190) (STJ29033)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U2OS cells using MEK6 antibody (STJ24469). Blue: DAPI for nuclear staining.

Anti-MAP2K6 antibody (1-334) (STJ24469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of L929 cells using MEK7 antibody (STJ24470) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MAP2K7 antibody (1-419) (STJ24470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of various lysates using MAP3K1 Rabbit pAb (STJ118497) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-MAP3K1 antibody (1400-1512) (STJ118497)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-MAP3K12 antibody (STJ500390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-MAP3K12 antibody [N377/20] (STJA0021420)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICC/IHC/WB

$461.03 - $1,954.13

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Anti-MAP3K12 antibody {Biotin} (STJ500391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-MAP3K12 antibody {FITC} (STJ500392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Immunocytochemistry analysis of MEKK2 (green) in Hela using MEKK2 antibody, and DAPI (blue)

Anti-MAP3K2 antibody (11-27 aa) [R06-2K7] (STJA0011589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$357.08

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Immunofluorescence analysis of U2OS cells using TAK1 Rabbit polyclonal antibody (STJ113923) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP3K7 antibody (110-325) (STJ113923)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of TAK1 in A431 using MAP3K7 antibody.

Anti-MAP3K7 antibody (550-606 aa) [R09-4G4] (STJA0014935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

$357.08

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Western blot analysis of TAK1 in Hela, A431 lysates using TAK1 antibody.

Anti-MAP3K7 antibody (593-606 aa) [R09-4B6] (STJA0012028)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

$357.08

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Immunofluorescence analysis of U2OS cells using MAP4K5 antibody (STJ117333) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MAP4K5 antibody (300-480) (STJ117333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] ERK2 Rabbit polyclonal antibody (STJ113009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAPK1 antibody (200-300) (STJ113009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (STJ24482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAPK1 antibody (200-300) (STJ24482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-MAPK1 antibody (C-Term) [M233] (STJA0005410)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

$527.18

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Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~42-44 kDa ERK/MAPK protein.

Anti-MAPK1/2 antibody (C-Term) (STJA0003630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$613.58

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Confocal imaging of HeLa cells using ERK1/2 Rabbit monoclonal antibody (STJ11102154, dilution 1:50) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-MAPK1/MAPK3 antibody (180-360) [S4MR] (STJ11102154)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of extracts of various cell lines, using ERK1/ERK2 antibody (STJ119100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAPK1/MAPK3 antibody (200-300) (STJ119100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Confocal immunofluorescence analysis of Hela cells using MAPK1/MAPK3 Rabbit polyclonal antibody (STJ119147) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-MAPK1/MAPK3 antibody (200-300) (STJ119147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of PC-12 cells using MAPK11 Rabbit polyclonal antibody (STJ110028) at a dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAPK11 antibody (264-364) (STJ110028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 200 Mu g extracts of U-87MG cells, using 3 Mu g p38 MAPK antibody (STJ116613). Western blot was performed from the immunoprecipitate using p38 MAPK antibody (STJ116613) at a dilution of 1:1000.

Anti-MAPK14 antibody (261-360) (STJ116613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-Mapk14 antibody (C-Term) [M138] (STJA0005503)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$532.58

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Anti-Mapk14 antibody (C-Term) [M548] (STJA0005504)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$431.33

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Anti-MAPK2 antibody (central Region) (STJA0005412)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$431.33

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Western blot analysis of various lysates, using ERK1 antibody (STJ11101698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK3 antibody (1-100) [S8MR] (STJ11101698)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunohistochemistry analysis of ERK1 in paraffin-embedded mouse heart using [KD Validated] ERK1 Rabbit polyclonal antibody (STJ24487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAPK3 antibody (1-378) (STJ24487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunofluorescence analysis of PC-12 using ERK1 Rabbit monoclonal antibody (STJ11104626) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK3 antibody (200-300) [S4626RM] (STJ11104626)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Rat

$174.83 - $775.58

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Immunofluorescence analysis of L929 cells using MAPK6 Rabbit polyclonal antibody (STJ117499) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAPK6 antibody (372-721) (STJ117499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options