Anti-EWSR1 antibody (1-300) (STJ115913)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EWSR1 (1-300) antibody for use in IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EWSR1 |
| Gene ID | 2130 |
| Uniprot ID | EWS_HUMAN |
| Immunogen Region | 1-300 |
| Immunogen Sequence | MASTDYSTYSQAAAQQGYSA YTAQPTQGYAQTTQAYGQQS YGTYGQPTDVSYTQAQTTAT YGQTAYATSYGQPPTGYTTP TAPQAYSQPVQGYGTGAYDT TTATVTTTQASYAAQSAYGT QPAYPAYGQQPAATAPTRPQ DGNKPTETSQPQSSTGGYNQ PSLGYGQSNYSYPQVPGSYP MQPVTAPPSYPPTSYSSTQP TSYDQSSYSQQNTYGQPSSY GQQSSYGQQSSYGQQPPTS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EWSR1 (NP_001156757.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylated.calmodulin-binding inhibits phosphorylation of Ser-266. Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8. |
| Function | Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. |
| Protein Name | Rna-Binding Protein EwsEws OncogeneEwing Sarcoma Breakpoint Region 1 Protein |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCell MembraneRelocates From Cytoplasm To Ribosomes Upon Ptk2b/Fak2 Activation |
| Alternative Antibody Names | Anti-Rna-Binding Protein Ews antibodyAnti-Ews Oncogene antibodyAnti-Ewing Sarcoma Breakpoint Region 1 Protein antibodyAnti-EWSR1 antibodyAnti-EWS antibody |
Information sourced from Uniprot.org